基于NF-κB信号通路的高压氧干预对BMSCs移植治疗脊髓损伤大鼠炎症因子及血糖水平的影响

Effects of hyperbaric oxygen intervention based on NF-κB signaling pathway on inflammatory factorsand blood glucose levels in rats with spinal cord injury treated by BMSCs transplantation

目的探讨基于核转录因子-κB(nuclear transcription factor-κB,NF-κB)信号通路的高压氧干预对骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)移植治疗脊髓损伤大鼠炎症因子及血糖水平的影响.方法SD大鼠40只随机分为假手术组、模型组、BMSCs组、BMSCs+高压氧组,每组10只,采用改良Allen打击法制备大鼠脊髓损伤模型,BMSCs组于造模1 h后尾静脉注射1 mL BMSCs悬液(3×10^6个);BMSCs+高压氧组于造模1 h后尾静脉注射1 mL BMSCs悬液(3×10^6个)+高压氧.术后第8天比较各组大鼠Basso-Beattie-Bresnahan(BBB)评分,比较各组大鼠脊髓组织病理学变化,检测并比较各组大鼠血清中白细胞介素1β(interleukin-1β,IL-1β)、肿瘤坏死因子α(tumor necrosis factorα,TNF-α)、血糖含量,检测并比较各组大鼠脊髓组织中NF-κB、核因子κB抑制蛋白α(inhibitorg protein of NF-κB-α,IκB-α)蛋白水平.结果各组大鼠BBB评分差异比较具有统计学意义(P<0.05);各组大鼠血清中TNF-α、IL-1β、血糖含量差异比较具有统计学意义(P<0.05),各组大鼠脊髓组织中NF-κB、IκB-α蛋白水平差异比较具有统计学意义(P<0.05).BMSCs+高压氧组较模型组、BMSCs组脊髓组织肿胀明显减轻,炎性细胞浸润明显减少,损伤区域结构改善更加明显.结论基于NF-κB信号通路的高压氧干预能够抑制BMSCs移植治疗脊髓损伤大鼠炎症因子表达,降低血糖水平,减轻脊髓损伤后病理变化,促进脊髓损伤修复.

Objective To investigate the effects of hyperbaric oxygen intervention based on nuclear transcription factor-κB(NF-κB)signaling pathway on inflammatory factors and blood glucose levels in rats with spinal cord injury treated by bone marrow mesenchymal stem cells(BMSCs)transplantation.Methods Forty SD rats were randomly divided into sham operation group,model group,BMSCs group,and BMSCs+hyperbaric oxygen group,10 rats in each group.Rats were made into models and received the corresponding treatments.On the 8th day after operation,various indexes were compared between groups,including hindlimb motor function assessed by Basso-Beattle-Bresnahan(BBB)scores,pathological changes of spinal cord tissue,the serum levels of interleukin-1β(IL-1β)and tumor necrosis factorα(TNF-α),blood glucose level,protein levels of NF-κB and inhibitory protein of NF-κB-α(IκB-α)in spinal cord tissue of each group.Results BBB scores showed statistic difference between the groups(P<0.05).Serum TNF-α,IL-1βand blood glucose levels showed statistic difference between the groups(P<0.05).The levels of NF-κB and IκB-αprotein in the spinal cord showed statistic difference between the groups(P<0.05).The pathological changes of spinal cord tissue in BMSCs+hypobaric oxygen group were significantly reduced compared with model group and BMSCs group.Conclusion Hyperbaric oxygen intervention based on NF-κB signaling pathway for rats with spinal cord injury can inhibit the expression of inflammatory cytokines,reduce blood glucose level,reduce pathological change,and promote the repair of spinal cord injury.