可利霉素对口腔鳞癌细胞生物学活性的影响

Inhibitory effect of carrimycin on biological activity of oral squamous cell carcinoma cells in vitro

目的:体外评价可利霉素对口腔鳞状细胞癌的抗肿瘤活性。方法:梯度浓度可利霉素分别处理口腔鳞状细胞癌细胞(HN6/HB96细胞系),采用CCK-8试剂盒检测细胞增殖,绘制生长曲线;采用划痕实验分析细胞迁移能力;采用平板克隆形成实验分析细胞克隆形成能力;采用流式细胞术分析细胞周期和细胞凋亡。采用SPSS 18.0软件包对数据进行统计学分析。结果:可利霉素浓度依赖性抑制HN6/HB96的体外增殖,随着药物浓度增加,细胞迁移和克隆形成能力显著下降(P<0.05);可利霉素使处理过的口腔鳞状细胞癌细胞G1期比例显著升高,S期和G2/M期比例显著下降,细胞周期阻滞于G1期(P<0.001)。可利霉素诱导口腔鳞状细胞癌细胞凋亡,随着药物浓度增加,细胞凋亡率显著上升(P<0.01)。结论:可利霉素体外抑制口腔鳞状细胞癌细胞的生物学活性,为可利霉素用于口腔鳞状细胞癌的治疗提供了实验依据。

PURPOSE:As a new macrolide antibiotic with independent intellectual property rights of China,carrimycin not only has good antibacterial effect on a variety of bacteria,but also has the function of regulating cell physiology and proliferation.The aim of this study was to investigate the anti-tumor effects of carrimycin on the biological activity of oral squamous cell carcinoma(OSCC)cells in vitro.METHODS:OSCC cells(HN6/HB96 cell lines)were treated with gradient concentration of carrimycin.Cell proliferation was detected using CCK-8 kit,based on which cell growth curve was drawn.Cell migration ability was analyzed by wound-healing assay.Cell clone formation ability was analyzed by clone formation assay.Cell cycle and apoptosis were analyzed by flow cytometry.SPSS18.0 software package was used for statistical analysis.RESULTS:Carrimycin concentration-dependently inhibited cellular proliferation of OSCC cells of HN6 and HB96 in vitro.With the increase of drug concentration,cell migration and clonal formation ability significantly decreased(P<0.05).The G1 phase ratio of OSCC cells treated with carrimycin significantly increased,while the S and G2/M phase ratio decreased,indicating that the cell cycle was arrested in G1 phase(P<0.001).The apoptosis rate of OSCC cells was significantly increased with the increase of drug concentration(P<0.01).CONCLUSIONS:The activity of OSCC cells can be effectively inhibited by carrimycin,which provides experimental evidence for the application of carrimycin in OSCC.