东海区黄鳍东方鲀寄生异尖线虫的鉴定及河鲀毒素检测

Identification of TTX Anisakis pegreffii parasites in Takifugu xanthopterus from the East China Sea

通过检查东海区283尾野生黄鳍东方鲀(Takifugu xanthopterus)样本,发现具有河鲀毒素的黄鳍东方鲀也受到线虫的感染。11月份黄鳍东方鲀体内异尖线虫感染情况最严重。通过形态学特征和线粒体DNA鉴定,黄鳍东方鲀组织内寄生的线虫为派氏异尖线虫(Anisakis pegreffii)。免疫组学分析发现异尖线虫体内含有河鲀毒素,进一步的高效液相色谱-质谱检测结果显示异尖线虫体内河鲀毒素含量为425 ng/g,这是首次发现寄生在东方鲀体内的线虫具有河鲀毒素。

We examined 283 wild Takifugu xanthopterus samples from the East China Sea to investigate the infection of Anisakis.It was found that the infection of Anisakis in T.xanthopterus was the highest in November.In situ photographing of the infected tissue of T.xanthopterus showed that the highest infection rate was as high as 60%in the liver and average infection intensity in the liver is 3 anisakises per fish.Identification by mitochondrial DNA molecules and morphologic features,the parasite of T.xanthopterus is Anisakis pegreffii.LC-MS/MS showed that the content of TTX in Anisakis was 425 ng/g.This is the first time that an anomalous nematode parasitic in T.xanthopterus has been found to have a tetrodotoxin.