藏仔猪粪便真菌菌群多样性分析

Fungal diversity in T ibetan piglet fecal samples

为探究西藏地区藏仔猪粪便中真菌菌群结构,本研究采用ITS1序列分析新生藏仔猪和断奶藏仔猪新鲜粪便中真菌的多样性。结果表明:从5头新生藏仔猪新鲜粪便中,共鉴定出5个门、13个纲、25个目、33个科和38个属的真菌;从5头断奶藏仔猪新鲜粪便中,共鉴定出5个门、12个纲、20个目、24个科和28个属的真菌。子囊菌门和担子菌门为优势门,占门级分类的86.35%;布勒担菌科、虫草科、丛赤壳科、曲霉科、丝孢酵母菌科、肉杯菌科、酵母菌科和毕赤酵母菌科为优势科,占科级分类的54.38%。随着藏仔猪的生长,其粪便中真菌菌群的数量和优势真菌的相对丰度均发生变化,这说明不同生长发育阶段会影响藏仔猪粪便中真菌的多样性。

ITS1 sequencing method was used to analyze the diversity of fungi in fresh feces of Tibetan newborn piglets and weaned piglets.Five phyla,13 classes,25 orders,33 families and 38 genera were discovered in feces of newborn Tibetan piglets.Five phyla,12 classes,20 orders,24 families and 28 genera were discovered in feces of weaned piglets.Ascomycota and Basidiomycota were dominant phyla;Bulleribasidiaceae,Cordycipitaceae,Aspergillaceae,Trichosporonaceae,Sarcoscyphaceae,Nectriaceae,Saccharomycetaceae and Pichiaceae were dominant families.Diversity of fungi and relative abundance of dominant fungi in feces varied with the growth of piglets,indicating that different growth stages could affect microflora in Tibetan piglet feces.