两种不同方法制备的黑木耳多糖性质比较

Optimization of preparation method and property analysis of Auricularia heimuer polysaccharide

本研究以黑木耳子实体为材料,对比了壳聚糖絮凝法制备的絮凝多糖HJD-1和传统水提醇沉法制备的醇沉多糖HJD-2的表观结构、α-葡萄糖甘酶抑制活性以及体外抑制肿瘤细胞增殖的活性。结果表明:(1)壳聚糖絮凝法制备粗多糖得率均值为4.76%,是醇沉法的2.17倍;醇沉法制备多糖的损失率为33.87%,是絮凝法的1.36倍;(2)絮凝多糖HJD-1和醇沉多糖HJD-2的表观评估及复溶性结果分析显示:絮凝多糖HJD-1为亮白色透明晶体,色泽均匀,颗粒规整;醇沉多糖HJD-2为棕褐色,颗粒状,有砂质感,前者相较后者的复溶性更好;(3)对α-葡萄糖甘酶抑制活性以及体外抗肿瘤能力结果分析表明:在相同浓度下,壳聚糖絮凝法制备黑木耳多糖对α-葡萄糖苷酶活性的抑制效果优于醇提法;絮凝多糖HJD-1对Hep G2细胞的增殖抑制作用强于醇沉多糖HJD-2。

The fruit bodies of Auricularia heimuer were used as the material to compare the apparent structure andα-glucosidase inhibitory activity as well as the activity of inhibiting tumor cell proliferation in vitro of the flocculating polysaccharide HJD-1 prepared by chitosan flocculation method and the alcohol precipitation polysaccharide HJD-2 prepared by traditional water extraction and alcohol precipitation method.The results showed that the average yield of crude polysaccharide prepared by chitosan flocculation method was 4.76%,being 2.17 times the yield of polysaccharides prepared by alcohol precipitation method;the loss rate of polysaccharide prepared by alcohol precipitation method was 33.87%,being 1.36 times that prepared by flocculation method.Apparent evaluation and resolubility analysis of flocculating polysaccharide HJD-1 and alcohol precipitation polysaccharide HJD-2 showed that flocculating polysaccharide HJD-1 was bright white transparent crystal with uniform color and regular particles,while alcohol precipitation HJD-2 was brown,granular,and sandy,and resolvability of the former is better than that of the latter.Analysis ofα-glucosidase inhibitory activity and anti-tumor ability indicated that at the same concentration,the inhibitory effect of polysaccharide prepared by chitosan flocculation onα-glucosidase activity was better than that prepared by alcohol extraction.The inhibition of proliferation of HepG2 cells by flocculating polysaccharide HJD-1 was stronger than that by alcohol precipitation polysaccharide HJD-2.