高产叶黄素小球藻(Chlorella sorokiniana)FZU60高盐度适应性驯化及表征

High-salinity adaptive domestication and characterization of high lutein producing microalgae Chlorella sorokiniana FZU60

提高微藻耐盐性可减少培养过程对淡水资源的依赖,从而降低微藻基产品的生产成本。对高产叶黄素的小球藻(Chlorella sorokiniana)FZU60进行高盐度适应性驯化,分析了驯化过程藻细胞生长及细胞组成的变化,并对比了耐盐株和原始株的叶黄素生产情况。结果表明,经过长期的高盐度适应性驯化培养,最终可获得在50 g/L盐度下正常生长的小球藻FZU60耐盐藻株。与原始株相比,耐盐藻株的细胞体积增大、藻体颜色加深,其油脂和蛋白质质量分数均有所提高,色素含量无显著变化,但碳水化合物含量降低。在30 g/L盐度下,耐盐株具有较高的生物量产率767.89 mg/(L·d)和叶黄素产率5.62 mg/(L·d),分别比原始株提高了20.34%和20.31%。筛选获得的小球藻FZU60耐盐藻株具有更高的叶黄素生产能力,可为利用海水进行微藻叶黄素生产及降低生产成本提供合适的藻种来源。

Improving the salt-tolerance of microalgae can reduce the dependence on fresh water resources in the culture process and thus reduce the production cost of microalgae-based products.The high-salinity adaptive domestication of high lutein producing microalgae Chlorella sorokiniana FZU60 was carried out,and the changes in cell growth and cell composition during adaptive domestication were analyzed.The results showed that a salt-tolerant strain growing well under 50 g/L salinity was obtained after high salinity adaptive domestication.The cell size of salt-tolerant strain was bigger,and the color was darker than that of wild strain.In addition,the mass fraction of lipid and protein of salt-tolerant strain was increased,and the mass fraction of pigment was similar,while the mass fraction of carbohydrate was decreased,compared with that of wild strain.At 30 g/L salinity,the salt-tolerant strain could reach higher biomass productivity of 767.89 mg/(L·d)and lutein productivity of 5.62 mg/(L·d),which were 20.34%and 20.31%higher than that of wild strain,respectively.Hence,the salt-tolerant strain obtained greater lutein production ability,which can provide a suitable algal species source for microalgae-based lutein production and reduce the production cost by using seawater.