摘要
为工业化控制黄曲霉毒素B 1(aflatoxin B 1,AFB 1)污染,筛选吸附率高、稳定性好、脱毒后具备食品安全的优势菌株,应用超高效液相色谱-三重四级杆串联质谱(ultra performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)技术检测菌株发酵液AFB 1残留量的方法,从35份采集自甘肃不同地域的西北酸菜样品中筛选到1株活菌脱毒率为66.41%、解吸后仍保留脱毒率为53.7%的菌株L34-2。菌株L34-2经形态学、生理生化及16S rRNA基因序列扩增测序,鉴定为布氏乳杆菌(Lactobacillus buchneri)。通过磷酸缓冲盐溶液(phosphate buffer saline,PBS)洗脱,体外模拟胃液消化,研究菌株-AFB 1复合体稳定性,结果表明,菌株L34-2吸附AFB 1具有较好的稳定性。
In order to solve the problem of industrial large-scale application of biological method to remove aflatoxin B 1(AFB 1),an AFB 1-adsorptive strain L34-2 was isolated from pickled vegetable in northwest China and its AFB 1 detoxification rate was 66.41%.Based on the morphological,physiological,biochemical analysis and sequence alignment of 16S rRNA,it was identified as Lactobacillus buchneri.The stability of the bacteria-AFB 1 complex was studied by phosphate buffer saline(PBS)elution and in vitro gastric juice digestion simulation.The results showed that it had good stability in adsorption of AFB 1.
作者
唐璎
孟宪刚
邓展瑞
马芮萍
黄佳
TANG Ying;MENG Xiangang;DENG Zhanrui;MA Ruiping;HUANG Jia(Gansu Province Product Quality Supervision and Inspection Research Institute,Lanzhou 730050,China;School of Chemical and Biological Engineering,Lanzhou Jiaotong University,Lanzhou 730070,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2020年第15期60-65,共6页
Food and Fermentation Industries
基金
甘肃省市场监督管理局科技计划项目(SSCJG-ZJ-201907)。
