螺旋筛板气升式反应器在真菌发酵中的应用

Application of airlift reactor with helical sieve plate in fungal fermentation

在发酵工业中,性能优良的反应器能够起到增加产量和提高能量利用效率的作用。新研发的带有螺旋筛板的新型气升式反应器(airlift reactor with helical sieve plate,ALR-HSP),已经在空气-水实验中证实了其优良的传质特性。为研究反应器综合性能,选取2种具有代表性的真菌(高山被孢霉和毕赤酵母)在ALR-HSP中进行通风培养,分析发酵过程参数和产物组成并与经典气升式反应器(conventional airlift reactor,CALR)进行比较。结果显示:在高山被孢霉培养中,ALR-HSP内菌体的对数生长期的最大比生长速率较CALR提高30%,发酵结束后脂肪酸总量提高约40%;毕赤酵母培养中,ALR-HSP内菌体对数生长期的最大比生长速率较CALR提高20%,发酵结束后菌体浓度增长约20%。且在低通风量条件下,达到相同k L a,ALR-HSP的功率消耗比CALR减少50%。显示出螺旋筛板气升式反应器在耗氧生物培养过程中的优越传质特性和节能效益。

High-performance reactors play an important role in increasing yield and energy efficiency in fermentation industries.A novel airlift reactor with helical sieve plate(ALR-HSP)developed in our laboratory had proven its excellent mass transfer characteristics in air-water system.To validate its comprehensive performance,two typical fungi(Mortierella alpine and Pichia pastoris)were selected and cultivated in ALR-HSP comparison to that of conventional airlift reactor(CALR).In the cultivation of M.alpine,the maximal specific growth rate during the logarithmic growth phase of ALR-HSP were 30%higher than that of CALR,and the total fatty acid increased by about 40%under the same culture conditions.,The maximum specific growth rate of P.pastoris during the logarithmic growth phase of ALR-HSP was 20%higher than that of CALR,and the concentration of biomass increased by about 20%.Under the condition of low gas flow rate,the power consumption of ALR-HSP was only about half of CALR when the same k L a was reached.These data show that the ALR-HSP had superior mass transfer characteristics and power saving performance during the process of aerobic biological cultivation.