假单胞菌噬菌体基因组学研究进展

Progress on phage genomics of Pseudomonas spp.

假单胞菌属(Pseudomonas spp.)是地球上重要的生态菌群之一,广泛分布于淡水、土壤等生态环境。假单胞菌噬菌体是以假单胞菌为宿主的病毒,不仅影响宿主的生存状况和进化过程,而且在生物物质循环和能量流动中扮演着重要角色。随着基因组测序技术的飞速发展,许多假单胞菌噬菌体的全基因组测序工作已经完成。截至2020年7月,GenBank收录的假单胞菌噬菌体基因组数有247条,占全部病毒基因组(10,069条)的2.45%。由于假单胞菌噬菌体基因组大小差异较大、遗传含量不同、基因组之间相似性较低,因此对假单胞菌噬菌体基因组的研究相对较少。本文主要对假单胞菌噬菌体基因组的特点、遗传多样性和功能基因方面的研究进行了综述,以期为理解细菌和噬菌体的对抗性共进化作用以及噬菌体的遗传进化提供参考。

Pseudomonas spp.are one of the most important ecological flora on the earth,widely distributed in freshwater,soil and other ecological environments.Pseudomonas phages are viruses hosted by Pseudomonas spp.,which not only affect the survival and evolution of the hosts,but also play important roles in biomass circulation and energy flow.With the rapid development of genome sequencing technologies,the whole genome sequences of many Pseudomonas phages have been completed.As of July 2020,247 Pseudomonas phage genomes were deposited in GenBank,accounting for 2.45%of the total 10,069 viral genomes.The genome sizes of Pseudomonas bacteriophages and the genetic contents are different,and the similarity between genomes is low,so the study on Pseudomonas bacteriophage genomes is relatively less.In this review,we summarize the characteristics,genetic diversity,and functional genes of Pseudomonas bacteriophages genomes in order to provide a reference for understanding the antagonistic coevolution of bacteria and phages and the genetic evolution of phages.