HPLC法测定不同年生野山参中9种人参皂苷含量

Determination of Nine Ginsenosides in Wild Ginseng of Different Ages by HPLC

目的建立测定不同年生野山参中人参单体皂苷含量的方法。方法采用高效液相色谱法(HPLC)进行测定,色谱柱为Thermo BDS HypersiL C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-水,梯度洗脱,流速为1.0mL/min,检测波长为203 nm,柱温为40℃,进样量为5μL。结果不同年生野山参中人参单体皂苷的含量为0.1449~6.9259 mg/g,该方法线性良好,r均大于0.9995,具有较高的精密度、稳定性和重复性,平均加样回收率为96.29~98.95%,RSD均小于2%(n=6)。结论本文所建立的含量测定方法简便易行、专属性强、准确可靠,为野山参的基础成分研究提供了参考依据,为野山参的质量控制奠定了基础。

Objective The HPLC method for content determination of ginsenosides in WiLd Ginseng of different ages was established in this paper.Methods The content of ginsenosides were determined by HPLC method.The analysis was performed on an Thermo BDS HypersiL C18 column(250 mm×4.6 mm,5μm)maintained at the temperature of 40℃ using acetonitrile-water as the mobile phase.Gradient elution was used in this determination with the flow rate of 1.0 mL/min.The detection wavelengths was set at 203 nm.The injection volume was 5μL.Results The content of ginsenosides in Wild Ginseng of different ages were in the range of 0.1449 and 6.9259 mg/g.Nine constituents showed good linear relationships within their own concentration ranges(r>0.9995).The method showed good precision,stability and repeatability.The average recoveries were found to be 96.29%~98.95% with the RSD all less than 2%(n=6).Conclusion The proposed method is simple,accurate and reproducible and can provide a reference for the basic constituents research and lay foundation for the quality control of Wild Ginseng.