碳纤维生物接触氧化法处理黑臭水体过程中的微生物多样性演变特征

Evolution Characteristics of Microbial Diversity During the Treatment of Black-odor Water by Carbon Fiber Bio-contact Oxidation

为了探讨碳纤维生物接触氧化法处理黑臭水体过程中,生长环境对细菌群落结构多样性特征演变的影响,利用高通量测序技术,对接种污泥以及设备内不同处理阶段的活性污泥、碳纤维膜上的细菌群落特征进行了研究。结果表明,随着挂膜的进行,设备内活性污泥的微生物种类逐渐减少,与接种污泥的细菌群落结构差异性越来越大,但是碳纤维膜E样品与稳定期D样品的细菌群落结构差异性较小。优势细菌菌群分析结果表明,在门类水平上达到20门以上,优势细菌种群以变形菌门、厚壁菌门、放线菌门、拟杆菌门的细菌为主,相对丰度约为75%~95%;纲类水平也达到20纲以上,主要以α-变形菌纲、β-变形菌纲、δ-变形菌纲、γ-变形菌纲为主。设备内活性污泥和碳纤维上的α-变形菌纲和γ-变形菌纲均高于接种污泥,梭菌纲含量低于接种污泥,说明采用活性污泥进行接种挂膜,细菌群落结构发生了较大变化。

In order to investigate the influence of the growth environment on the evolution of the structural diversity characteristics of the bacterial community during the treatment of black-odor water by carbon fiber bio-contact oxidation,this paper studies the characteristics of bacterial community on inoculated sludge,in-equipment activated sludge and carbon fiber membrane by high-throughput sequencing techniques.The results show that with the progress of biofilm culturing,the microbial species of activated sludge in the equipment gradually decreased,the diversity of bacterial community structure increased compared with inoculated sludge.The difference in bacterial community structure between the carbon fiber membrane D sample and E sample at stable phase was small.The analysis results of dominant bacterial flora show that there were 20 phylum found in the samples.The dominant microbial populations were Proteobacteria,Pachyphyta,Actinomycetes and Bacteroides with their relative abundance of about 75%~95%.At the class level,more than 20 classes were found in the samples,the dominant microbial populations were Alphaproteobacteria,Betaproteobacteria,Deltaproteobacteria and Gammaproteobacteria.The Alphaproteobacteria and Gammaproteobacteria on activated sludge and carbon fiber in the equipment were higher than those in the inoculated sludge,and the clostridia was lower than that in the inoculated sludge,indicating that the bacterial community structure had undergone significant changes after inoculated biofilm culturing with activated sludge.