丁酸钠、益生菌和酶制剂对白羽肉鸡生长性能、肠道健康及肠道微生物组成的影响

Effect of sodium butyrate, probiotics and enzymes on growth performance, intestinal health and microbial composition in white feather broiler

试验探讨丁酸钠及其与益生菌和酶制剂联合添加对肉鸡生长性能、肠道形态结构、抗炎抗氧化功能以及微生物组成的影响。试验选择2100刚出雏的白羽肉仔鸡分为3组:对照组,饲喂基础日粮;丁酸钠组,第1~14 d添加0.1%的丁酸钠;复合组,第1~14 d添加0.1%的丁酸钠,全程添加0.02%益生菌和0.05%酶制剂。结果表明,与对照组相比,丁酸钠组和复合组肉鸡平均日增重和平均日采食量显著增加(P<0.05),而料重比显著降低(P<0.05);丁酸钠组和复合组肉鸡回肠绒毛高度显著增加(P<0.05)、肠道超氧化物歧化酶活性显著升高(P<0.05),而丙二醛含量显著降低(P<0.05);复合组肉鸡肠道总抗氧化酶和谷胱甘肽还原酶活性以及谷胱甘肽浓度显著升高(P<0.05);丁酸钠组肉鸡肠道IL-1βmRNA表达量显著降低(P<0.05),而复合组肉鸡肠道IL-1β和IL-6 mRNA表达量均显著降低(P<0.05)。微生物组成分析发现,丁酸钠组和复合组肉鸡微生物丰富度显著高于对照组(P<0.05)。由此可见,添加丁酸钠或者联合添加丁酸钠、益生菌和酶制剂显著提高白羽肉鸡生长性能,维持肠道形态结构完整性,缓解炎症和氧化应激以及影响肠道微生物组成。

The study was to evaluate the effect of sodium butyrate and its combination with probiotics and enzymes on growth performance,intestinal morphology,expression of inflammatory cytokines and antioxidant enzyme genes and microbial composition in white feather broilers.2100 white feather broilers were selected and randomly assigned to three treatment groups:control group(CON),broilers fed with basic diet.Sodium butyrate group(DSN),broilers fed with basic diet supplemented with 0.1%sodium butyrate during day 1~14.The compound group(FN),broilers fed with basic diet supplemented with 0.1%sodium butyrate during day 1~14,and with 0.02%probiotics and 0.05%enzymes throughout the experiments.The results showed that compared with broilers in CON group,the daily weight gain and daily feed intake of broilers in DSN and FH group were significantly increased(P<0.05),while the feed to gain ratio was significantly decreased(P<0.05).Compared with broilers in CON group,villi height was significantly increased in DSN and FH group(P<0.05),intestinal total antioxidant enzyme and glutathione reductase activity and glutathione concentration of broilers in FH group were significantly increased(P<0.05).Compared with broilers in CON group,intestinal superoxide dismutase activity of broilers in DSN and FH group was significantly increased(P<0.05),while malondialdehyde content was significantly decreased(P<0.05).Compared with broilers in CON group,intestinal IL-1βmRNA expression in broilers in DSN group was significantly decreased(P<0.05),while expression levels of intestinal IL-1βand IL-6 mRNA in broilers in FH group were significantly decreased(P<0.05).In addition,microbial composition analysis showed that the richness of microbiota in cecum of broilers in DSN and FH group was significantly higher than that in CON group(P<0.05).In conclusion,dietary sodium butyrate or its supplementation in combination with probiotics and enzymes can significantly improve the growth performance,maintain the intestinal morphology integrity,alleviate intestinal inflammation and oxidative stress,and affect the intestinal microbial composition in white feather broilers.