OIP5-AS1和miR-410在胶质瘤中的表达及调控机制

The expression and regulation mechanism of OIP5-AS1 and miR-410 in glioma tissues

目的对比OIP5-AS1和miR-410在胶质瘤与正常脑组织中表达的差异并探讨OIP5-AS1与miR-410的关系及作用机制。方法收集胶质瘤患者标本65例,脑组织标本10例作为对照组。通过qRT-PCR方法检测2组标本中OIP5-AS1和miR-410的表达情况并进行比较。通过Starbase 2.0预测:OIP5-AS1与miR-410的关系。通过双荧光素酶报告基因检测miR-410和OIP5-AS1的靶向关系。结果与正常组织比较,胶质瘤组织中OIP5-AS1的表达水平明显升高,miR-410的表达明显降低(P<0.05)。OIP5-AS1在高级别胶质瘤组的表达显著高于低级别组,miR-410的表达趋势相反。相关性分析结果示,OIP5-AS1表达与miR-410表达呈负相关。Starbase 2.0预测:OIP5-AS1是调节miR-410的分子海绵。双荧光素酶活性测定:miR-410 mimics显著下调OIP5-AS1-wt的荧光素酶活性(P<0.05)。结论胶质瘤组织中OIP5-AS1的表达水平明显升高,miR-410的表达明显降低,且OIP5-AS1和miR-410的表达与胶质瘤的病理分级相关。OIP5-AS1表达与miR-410表达呈负相关,两者存在靶向调节关系。

Objective To investigate the differences of expression levels of OIP5-AS1 and miR-410 in glioma tissues and normal brain tissues,and to explore the correlation between the expression of OIP5-AS1 and miR-410 as well as their action mechanism.Methods A total of 65 specimens of glioma tissues were collected as glioma group,and 10 specimens of normal brain tissues were collected as control group.The expression levels of OIP5-AS1 and miR-410 in glioma and normal brain tissues were detected by qRT-PCR.And the correlation between OIP5-AS1 and miR-410 was analyzed by means of Starbase 2.0.Moreover the targeting relationship between miR-410 and OIP5-AS1 was analyzed by Dual-luciferase reporter assay.All the data were processed and analyzed by using the statistics software SPSS 20.0.Results As compared with those in normal brain tissues,the expression levels of OIP5-AS1 were upregulated,however,the expression levels of miR-410 were downregulated in glioma tissues.The expression levels of OIP5-AS1 in high-grade glioma group were significantly higher than those in low grade glioma group,however,the expression levels of miR-410 in high-grade glioma group were significantly lower than those in low grade glioma group.The correlation analysis showed that the expression levels of OIP5-AS1 were closely negatively correlated with those of miR-410.The Starbase 2.0 prediction indicated that OIP5-AS1 was a molecular sponge that regulated miR-410.According to the dual-luciferase activity assay,miR-410 mimics significantly down-regulated the luciferase activity of wild-type OIP5-AS1-wt.Conclusion The expression levels of OIP5-AS1 were upregulated in glioma tissues,however,the expression levels of miR-410 were downregulated in glioma tissues.Moreover the expression levels of OIP5-AS1 and miR-410 are significantly associated with the pathological grading of glioma,and the expression levels of OIP5-AS1 negatively correlated to those of miR-410,and there is a targeting regulation correlation between OIP5-AS1 and miR-410.