人参皂苷Rg1对缺血性脑卒中大鼠的保护作用及其机制研究

Protective effect of ginsenoside Rg1 on ischemic stroke rats and its mechanism

目的探讨人参皂苷Rg1对缺血性脑卒中大鼠的保护作用及其对丝裂原活化蛋白激酶(MAPK)/核转录因子κB(NF-κB)信号通路的调控机制。方法按照体重将大鼠随机分为4组:假手术组、模型组、实验组和抑制剂组,每组12只。用线栓法建立永久性局灶脑缺血大鼠模型。造模前48 h灌胃给药,实验组给予10 mg·kg^-1人参皂苷Rg1,抑制剂组给予10 mg·kg^-1MAPK/NF-κB通路抑制剂,假手术组和模型组给予等体积的0.9%NaCl,连续给药7 d。以氯化三苯基四氮唑染色检测大鼠脑梗死体积,以蛋白质印迹法检测p-ERK1/2和NF-κB的表达。结果假手术组、模型组、实验组和抑制剂组的大鼠脑梗死体积百分率分别为0,(45.35±5.13)%,(22.33±1.26)%和(22.85±1.35)%;这4组的p-ERK1/2的相对表达分别为1.00±0.05,4.56±0.15,2.25±0.32和2.22±0.35;这4组的NF-κB的相对表达分别为0.97±0.04,4.73±0.21,2.33±0.08和2.37±0.11。上述指标:模型组与假手术组相比,差异均有统计意义(均P<0.05);实验组、抑制组与模型组相比,差异均有统计意义(均P<0.05);实验组和抑制剂组相比,差异均无统计学意义(均P>0.05)。结论人参皂苷Rg1抑制MAPK/NF-κB信号通路,从而发挥对缺血后的脑组织的保护作用。

Objective To explore the protective effect of ginsenoside Rg1 on ischemic stroke rats and its regulatory mechanism of mitogen-activated protein kinases(MAPK)/nuclear transcription factorκB(NF-κB)signaling pathway.Methods Rats were randomly divided into four groups according to weight:sham operation group,model group,experimental group and inhibitor group.The rats model with permanent focal cerebral ischemia was established by thread embolism method.Foruty-eight h before the operation,the experimental group was given 10 mg·kg^-1 ginsenoside Rg1,the inhibitor group was given 10 mg·kg^-1 MAPK/NF-κB pathway inhibitor,and the sham group and model group were given equal volumes of normal saline,for 7 d.Triphenyltetrazolium chloride staining was used to detect cerebral infarction volume,and Western blot was used to detect the expression of p-ERK1/2 and NF-κB.Results Cerebral infarction volume(%)in sham operation group,model group,experimental group and inhibitor group were respectively 0,(45.35±5.13)%,(22.33±1.26)%,(22.85±1.35)%;the relative expression of p-ERK1/2 in the four groups were respectively 1.00±0.05,4.56±0.15,2.25±0.32 and 2.22±0.35;the relative expression of NF-κB in the four groups were respectively 0.97±0.04,4.73±0.21,2.33±0.08 and 2.37±0.11.Comparison between model group and sham operation group,the differences of the factors were significant(all P<0.05);comparison between experimental group,inhibitor group and model group,the differences of the factors were significant(all P<0.05);comparison between experimental group and inhibitor group,the differences of the factors were not significant(all P>0.05).Conclusion Ginsenoside Rg1 can inhibit the MAPK/NF-κB signaling pathway,thereby play a protective role in the brain tissue after ischemia.