病毒NS1抗原捕获酶联免疫吸附试验在登革热筛查中的应用价值

Application value of virus NS1 antigen capture enzyme linked immunosorbent assay in screening of dengue fever

目的研究病毒NS1抗原捕获酶联免疫吸附试验(NS1-ELISA)在登革病毒感染前期筛查和诊断中的应用价值。方法选取2014年10月至2019年10月我院收治的16895例疑似登革病毒感染患者作为研究对象,通过胶体金免疫层析法、荧光聚合酶链式反应(qPCR)及NS1-ELISA检测患者血清进行筛查。比较三种方法的检测结果及检测效能。结果胶体金免疫层析法的检出率(70.00%)高于其他两种方法(qPCR的检出率为65.31%;NS1-ELISA的检出率为65.00%)。NS1-ELISA的灵敏度、特异度、约登指数略高于胶体金免疫层析法和qPCR。结论NS1-ELISA对于早期筛查登革病毒感染具有重要的价值,相比于其他两种诊断方法的效果较好,可为临床检查和诊断提供一定的帮助。

Objective To study the application value of NS1 antigen capture enzyme linked immunosorbent assay(NS1-ELISA)in pre-infection screening and diagnosis of dengue virus.Methods A total of 16895 patients with suspected dengue virus infection admitted in our hospital from October 2014 to October 2019 were selected as the research objects.The serum samples were detected by colloidal gold immunochromatography,fluorescence polymerase chain reaction(qPCR)and NS1-ELISA.The detection results and detection efficiency of the three methods were compared.Results The detection rate of colloidal gold immunochromatography(70.00%)was higher than that of the other two methods(the detection rate of qPCR was 65.31%;the detection rate of NS1-ELISA was 65.00%).The sensitivity,specificity and Youden index of NS1-ELISA were slightly higher than those of colloidal gold immunochromatography and qPCR.Conclusion NS1-ELISA has an important value for early screening of dengue virus infection,compared with other two diagnostic methods,NS1-ELISA has better effect and can provide certain help for clinical examination and diagnosis.