MGO-MI纳米复合材料对乏氧癌细胞的放化疗增敏作用

Chemoradiotherapy Sensitization of MGO-MI Nanocomposites for Hypoxic Cancer Cells

利用甲硝唑(metronidazole,MI)作为缺氧响应成分,使用分步合成法,制备磁性氧化石墨烯-甲硝唑(magnetic graphene oxide-metronidazole,MGO-MI)纳米复合材料,运用透射电子显微镜(TEM),动态光散射(DLS),X射线光电子能谱(XPS)和傅里叶红外变换光谱(FT-IR)等技术对其进行表征,并将得到的MGO-MI纳米复合材料与两种非小细胞肺癌细胞(H1975和A549)共同培养,从生物细胞层面评估其毒性、缺氧敏感性以及放射增敏作用。结果显示,在常氧情况下,一定浓度(0~100μg/mL)的MGO-MI纳米复合材料对两种细胞都没有表现出明显的毒性,但是缺氧情况下,MGO-MI纳米复合材料的毒性显著提高;该纳米复合材料还能有效增强X射线对两种癌细胞的杀伤作用,显著提高放疗效果,可望用于临床乏氧癌细胞放化疗。

With metronidazole(MI) as a hypoxia-responsive component, a step-by-step synthesis method was used to prepare magnetic graphene oxide-metronidazole(MGO-MI) nanocomposites. Moreover, the characterizations of the nanocomposites were conducted using transmission electron microscopy(TEM), dynamic light scattering(DLS), X-ray photoelectron spectroscopy(XPS) and Fourier transform infrared spectroscopy(FT-IR). Subsequently, the obtained MGO-MI nanocomposites were co-cultured with two kinds of non-small cell lung cancer cells(H1975 and A549) to evaluate the cytotoxicity, hypoxia sensitivity and radiation enhancement effect of the nanocomposites at the biological cell level. The results show that under normoxia conditions, the cytotoxicity of the nanocomposites to both cells in a certain concentration range(0~100 μg/mL) is slight, while it is significantly increased under hypoxia conditions. In addition, the nanocomposites can also effectively enhance the suppression effect of X-ray on both cells,significantly improve the efficiency of radiation,and is expected to be used in clinical chemoradiotherapy of hypoxic cancer cells.