利用CRISPR/Cas9系统快速建立APPswe/PS1dE9双转基因阿尔茨海默病小鼠模型

Establishment of APPswe/PS1dE9 double transgenic mouse models of Alzheimer’s disease by using CRISPR/Cas9 system

目的利用CRISPR/Cas9系统构建APPswe/PS1dE9双转基因阿尔茨海默病(AD)小鼠模型,为开展AD的病因治疗提供载体。方法采用CRISPR/Cas9系统,选择H11作为插入位点,将有活性的sgRNA、Cas9 RNA和含有目标同源重组序列的载体显微注射入C57BL/6品系野生雌性小鼠的受精卵中,鉴定得到的F0代阳性小鼠再与C57BL/6野生小鼠交配,从而获得F1代小鼠。对鉴定得到的F1代杂合子小鼠,选择来自同一只F0代小鼠基因型一致的F1代杂合子小鼠,达到性成熟后进行同胞交配,获得F2代小鼠。采用PCR方法鉴定F2代小鼠的基因型结果,采用Western blot检测APP和PS1在F2代小鼠脑组织中的蛋白表达水平。结果27只F2代小鼠经PCR鉴定基因型,5只F2代小鼠出现约344 bp和608 bp的目的基因条带,表明成功构建出转入APP和PS1基因的APPswe/PS1dE9双转基因AD小鼠。Western blot检测显示APPswe/PS1dE9双转基因AD小鼠的APP和PS1的表达水平均明显高于同窝野生型小鼠。结论利用CRISPR/Cas9系统可成功构建APPswe/PS1dE9双转基因AD小鼠。

Objective To establish APPswe/PS1dE9 double transgenic mouse model of Alzheimer’s disease(AD)by using CRISPR/Cas9 system.Methods With CRISPR/Cas9 system,H11 was selected as the insertion site,the active sgRNA,cas9 RNA and the vector containing the target homologous recombination sequence were microinjected into the fertilized eggs of C57BL/6 strain mice to obtain the gene modified primary mice;the double transgenic F1 mice was obtained by mating with wild mice.After sib mating,five APPswe/PS1dE9 transgenic homozygotes were obtained.PCR was used to identify the genotypes of double transgenic mice,and Western blot was used to detect the protein expression of APP and PS1 in brain tissue of mice.Results After PCR amplification,the target gene bands of 344 bp and 608 bp were found in transgenic AD mice,indicating that the transgenic mice were successfully generated.Western blot analysis showed that the expression of APP and PS1 in transgenic AD mice was significantly higher than that of wild-type mice in the same nest.Conclusion Using CRISPR/Cas system,APPswe/PS1dE9 double transgenic AD mice were successfully generated,which can be used for the etiology treatment study of AD.