广枣总黄酮干预早期激素性股骨头坏死的作用及其机制

Effect of total flavones of fructus chorspondiatis on early steroid-induced femoral head necrosis and its mechanism

目的探讨蒙药广枣总黄酮(TFFC)对早期激素性股骨头坏死兔细胞凋亡的影响及其作用机制。方法将34只购自西安市迪乐普生物资源开发有限公司的新西兰雄性兔随机分为3组:模型组和治疗组各12只,对照组10只,采用核磁共振(MRI)、病理组织学以及免疫组织化学来验证TFFC治疗股骨头坏死的作用。计量资料组间比较为t检验,计数资料组间比较为χ2检验。结果MRI结果显示TFFC可改善股骨头坏死区域影像学改变;病理组织学显示:治疗组兔股骨头坏死发生率(45.46%)明显低于模型组(80.00%)(χ2=13.230,P<0.05),且治疗组的空骨陷窝率明显低于模型组[(16.36±2.74)%比(22.35±3.15)%,χ2=4.950,P<0.05],差异有统计学意义;与对照组比较,模型组B细胞淋巴瘤/白血病-2(bcl-2)基因阳性细胞表达率明显下降[(13.04±2.05)%比(9.71±1.27)%,χ2=4.960,P<0.05],bcl-2相关X蛋白(bax)阳性细胞表达率明显增加[(11.51±0.63)%比(23.96±2.72)%,χ2=15.460,P<0.05],差异有统计学意义;而治疗组与模型组比较,bcl-2阳性细胞表达率明显升高[(21.49±1.16)%比(9.71±1.27)%,χ2=12.490,P<0.05],bax阳性细胞表达率明显降低[(19.00±1.61)%比(23.96±2.72)%,χ2=6.370,P<0.05],差异有统计学意义。结论TFFC可能通过上调bcl-2、下调bax的抗凋亡作用来干预早期激素性股骨头坏死。

Objective To investigate the effect of mongolian medicine total flavones of fructus chorspondiatis(TFFC)on apoptosis of rabbits with early steroid-induced femoral head necrosis and its mechanism.Methods A total of 34 New Zealand male rabbits purchased from Xi’an Dilepu Biological Resources Development Co.,Ltd.were randomly divided into 3 groups:model group(n=12),treatment group(n=12)and control group(n=10).Nuclear magnetic resonance(MRI),histopathology and immunohistochemistry were used to verify the efficacy of TFFC in the treatment of femoral head necrosis.T test and Chi-square test were used for the comparison between the two groups.Results MRI results showed that TFFC could improve the imaging changes of femoral head necrosis.Histopathology showed that the incidence of femoral head necrosis in the treatment group(45.46%)was significantly lower than that in the model group(80.00%)(χ2=13.230,P<0.05),and the rate of empty bone lacuna in the treatment group was significantly lower than that in the model group[(16.36±2.74)%vs.(22.35±3.15)%,χ2=4.950,P<0.05].As compared with the control group,the expression rate of B-lymphoma-2 gene(bcl-2)positive cells in the model group decreased significantly[(13.04±2.05)%vs.(9.71±1.27)%,χ2=4.960,P<0.05],and that of B lymphoma-2 associated X gene(bax)positive cells increased significantly[(11.51±0.63)%vs.(23.96±2.72)%,χ2=15.460,P<0.05].As compared with the model group,the expression rate of bcl-2 positive cells in the treatment group increased significantly[(21.49±1.16)%vs.(9.71±1.27)%,χ2=12.490,P<0.05],and that of bax positive cells decreased significantly[(19.00±1.61)%vs.(23.96±2.72)%,χ2=6.370,P<0.05].Conclusion TFFC can intervene early steroid-induced femoral head necrosis by up-regulating bcl-2 and down-regulating bax.