基于气相色谱-质谱联用的血府逐瘀汤治疗大鼠颅脑损伤的血浆代谢组学研究

Plasma Metabolomics Study of Effect of Xuefu Zhuyu Decotion on Rats Suffering Traumatic Brain Injury Based on Gas Chromatography-Mass Spectrometry

该文研究了血府逐瘀汤对颅脑损伤大鼠血浆代谢组的影响,采用气相色谱-质谱联用(GC-MS)技术对治疗组、模型组以及假手术组的血浆代谢物进行检测,定性定量分析了43种重要的代谢物。模型组和假手术组的t检验(t-test)结果显示13种代谢物存在显著差异。正交偏最小二乘-判别分析(OPLS-DA)分析结果也显示模型组和假手术组代谢差异明显。结合变量的投影重要性指标(VIP)及t检验结果,筛选出乳酸、组氨酸、棕榈酸、色氨酸、亚油酸、油酸、硬脂酸作为生物标志物。观测7种标志物在治疗组中第1、3、7、14 d的变化情况。结果显示:治疗组中乳酸、组氨酸、棕榈酸、亚油酸、硬脂酸的相对浓度逐渐降低,色氨酸的相对浓度先降低后升高,油酸的相对浓度先升高后降低,且7种代谢物的相对浓度在第14 d时均接近于假手术组的水平。表明血府逐瘀汤对颅脑损伤具有一定的治疗作用,乳酸、组氨酸、棕榈酸、色氨酸、亚油酸、油酸、硬脂酸7种代谢物可以作为生物标志物监测颅脑损伤的治疗效果及恢复情况。

This study aimed at the investigation of effect of Xuefu Zhuyu decotion(XFZY) on rats with traumatic brain injury(TBI) in plasma metabolomics.The plasma samples from the treatment group,the model group as well as the sham group were analyzed by gas chromatography-mass spectrometry(GC-MS).Forty-three important metabolites were qualitatively and quantitatively analyzed.t-test was employed for the model group and the sham group,and 13 metabolites were found with significant differences.Orthogonal partial least squares-discriminant analysis(OPLS-DA) was performed on the model group and the sham group.The obvious classification tendency was observed between the two groups.Integrating the variable importance in projection(VIP) and the results of T-test,lactic acid,histidine,palmitic acid,tryptophan,linoleic acid,oleic acid,and stearic acid were selected as biomarkers.The treatment group was gavaged with XFZY,and seven biomarkers were used to observe the metabolite changes in treatment groups experiencing the treatment after 1,3,7 and 14 days,respectively.It was found that the relative concentrations of lactic acid,histidine,palmitate,linoleic acid,and stearic acid decreased gradually.The relative concentration of tryptophan decreased at first,and then increased,while the relative concentration of oleic acid showed an opposite trend.A consensus is emerging about the relative concentration of seven metabolites between the sham group and treatment group on day 14.Experimental results showed that XFZY had a good therapeutic effect on TBI.Meanwhile,those metabolites,i.e lactic acid,histidine,palmitic acid,tryptophan,linoleic acid,oleic acid and stearic acid could be used as the biomarkers for monitoring the therapeutic effect and recovery of TBI.