番石榴叶炮制品质量标准研究

Study on Quality Standard of Processed Product of Psidium guajava Leaves

目的:建立番石榴叶炮制品质量标准,提高其质量控制水平。方法:观察番石榴叶炮制品药材粉末的显微鉴别特征,采用TLC对番石榴叶炮制品进行定性鉴别;采用药典方法测定其水分、灰分和浸出物,采用HPLC对其有效成分槲皮素进行含量测定。结果:对番石榴叶炮制品的显微特征进行了描述,番石榴叶炮制品薄层色谱斑点清晰,分离度好;暂定番石榴叶炮制品药材水分、总灰分及酸不溶性灰分分别不得过12%、7%、0.3%,醇溶性浸出物应不少于26.0%;番石榴叶炮制品中槲皮素的含量应不的少于0.05%。结论:该研究所建立的方法准确可靠,可用于番石榴叶炮制品质量控制和评价。

Objective To establish the quality standard of processed Psidium guajava leaves,and improve its quality control level.Methods To observe the microscopic characteristics of processed Psidium guajava leaves.Qualitative identification of processed Psidium guajava leaves.were identified by characters and TLC.the moisture,ash and extractives were detected according to the pharmacopeia methods.High performance liquid chromatography(HPLC) was used for the content determination.Results The TLC showed the spots developed clearly and the separation was good.The moisture,total ash and acid insoluble ash of processed Psidium guajava leaves were not more than12% 、7% 、0.3%,respectively.Alcohol soluble extract should be no less than 26.0%.The Quercetin of processed Psidium guajava leaves were not less than 0.05%.Conclusion The method established by this research institute was accurate and reliable,and could be used for quality control and evaluation of processed Psidium guajava leaves.