甘蔗CP84-1198×ROC22杂交群体抗褐锈病Bru1基因分子检测

Molecular Detection of Sugarcane Brown Rust Resistance Gene Bru1 in CP84-1198×ROC22 Population

为了更好地改良ROC22的农艺性状,本研究以ROC22为父本,CP84-1198为母本,构建了CP84-1198×ROC22杂交群体(1100个株系)。从杂交后代中挑取优良个体材料,以褐锈病抗性为指标,随机挑取280个株系苗期的叶片,运用CTAB方法提取甘蔗基因组DNA,以R12H16为标记用普通PCR技术对该群体各个株系进行锈病抗性基因Bru1检测,结果发现该杂交群体中156个(55.71%)株系检测到了该基因,含有与不含有抗锈病基因Bru1符合理论比1∶1,研究结果表明ROC22含有单个抗锈病Bru1等位基因,CP84-1198不含有抗锈病Bru1等位基因。以期能为深入开展甘蔗群体抗褐锈病育种、选育和推广优良抗性品种(系)提供材料基础。

In order to improve the agronomic traits of ROC22,we used ROC22 as male parent and CP84-1198 as female parent to construct hybrid population(1100 lines).To select more useful materials in this population,lines brown rust resistance was an importance factor in this study.280 lines of leaves were randomly selected in seedling stage,DNA was extracted by CTAB,rust resistance gene Bru1 was detected by PCR amplification with marker R12H16.The results showed that 156(55.71%)lines of the hybrid population was positive in the detection,the ratio of Bru1 gene positive and negative was 1∶1,indicating that ROC22 contained a single Bru1 allele and CP84-1198 did not contain Bru1 allele.The results provided the material basis for the further development of brown rust resistance breeding in sugarcane population,breeding and promotion of resistant lines.