邻苯三酚自氧化法对茶叶中茶多酚的抗氧化性能应用研究

Pyrogallol Autoxidation and Its Application to Tea Polyphenols Activity Assay

为研究本地不同类型茶叶中茶多酚的抗氧化能力,以梵净山绿茶、梵净山小酱白茶、铜仁苦丁茶、石阡苔茶(红茶)为原料,在邻苯三酚浓度0.4 mg/mL,磷酸盐(phosphate beffered soiution,PBS)缓冲液pH=8.0,邻苯三酚溶液与PBS缓冲液(pH=8.0)体积比0.1∶5,反应时间25min的条件下进行紫外光谱扫描,通过邻苯三酚自氧化体系评价其抗氧化活性。结果表明:茶多酚清除超氧自由基能力(IC50)分别为0.08、0.098、0.119、0.136 mg/mL。由此得知,不同类型茶叶其茶多酚抗氧化能力不同,绿茶最高,其次是小酱白茶、再次是苦丁茶和红茶,且抗氧化能力与茶多酚浓度呈良好的线性关系,这为后期的应用研究提供一定的理论依据。

In order to study the local different types of tea in tea polyphenols antioxidant capacity,this paper,taking Fanjing mountains green tea,white tea Fanjing mountains small sauce,tongren bitter butyl tea and Shi Qian moss tea(black tea)as raw materials,in the development of three phenol concentration of 0.4 mg/mL,PBS buffer pH=8.0,the development of three phenol with PBS buffer solution(pH=8.0)volume ratio=0.1:5,under the condition of the reaction time of 25 min,ultraviolet spectrum scanning through the development of three phenol autoxidation system to evaluate its antioxidant activity.The results showed that:the ability of tea polyphenols to scavenge superoxide radicals(IC50)was 0.08,0.098,0.119mg/mL and 0.136 mg/mL,respectively.According to this,different types of tea had different antioxidant capacity of tea polyphenols,and green tea had the highest antioxidant capacity,followed by xiaojiang white tea,kuding tea and black tea,and the antioxidant capacity had a good linear relationship with the concentration of tea polyphenols,which provided certain theoretical basis for later application research.