miRNA-29c在喉癌组织中的表达及对喉癌Hep-2细胞增殖、侵袭的影响

Expression of miRNA-29c in the tissue of laryngeal carcinoma and its effects on proliferation and invasion of laryngeal carcinoma Hep-2 cells

目的观察miRNA-29c在喉癌组织和喉癌Hep-2细胞株中的表达情况,研究其与肿瘤临床分期、淋巴转移及预后等临床病理参数的关系,并探讨miRNA-29c对喉癌Hep-2细胞增殖、侵袭的影响。方法选取2006年1月~2016年12月在我院确诊的86例喉癌患者的蜡块标本,利用实时荧光定量聚合酶链式反应检测喉癌组织和癌旁正常喉黏膜上皮组织中miRNA-29c的表达,并分析其与临床病理学特征的关系;通过Q-RT-PCR检测转染后每组细胞中miRNA-29c的表达;CCK-8检测细胞增殖能力变化情况;Transwell实验检测跨膜细胞数量的变化。结果miRNA-29c在喉癌组织中相对表达量明显低于癌旁组织(P<0.01),其表达量与不同临床分型、TNM分期、淋巴转移密切相关(P<0.05);转染miRNA-29c模拟物组细胞中的miRNA-29c表达水平显著高于无关序列组和空白对照组(P<0.01),并可抑制喉癌Hep-2细胞的增殖和侵袭力(P<0.01)。结论在喉癌中,miRNA-29c是一个抑制性miRNA,miRNA-29c低表达可能是影响喉癌预后的重要因素;过表达miRNA-29c可显著降低喉癌Hep-2细胞的增殖和侵袭能力,miRNA表达水平的下调可能参与了喉癌的发生发展及侵袭转移过程。

Objective To observe the expression of miRNA-29c in the tissue of laryngeal carcinoma and laryngeal carcinoma Hep-2 cell line,to study its relationship with clinical pathological parameters such as clinical staging,lymphatic metastasis and prognosis,and to investigate the effect of miRNA-29c on proliferation and invasion of laryngeal carcinoma Hep-2 cells.Methods Paraffin-embedded specimens of a total of 86 patients with laryngeal cancer who were diagnosed in our hospital from January 2006 to December 2016 were selected.Real-time fluorescence quantitative polymerase chain reaction was used to detect the expression of miRNA-29c in laryngeal carcinoma tissues and adjacent normal laryngeal mucosa epithelial tissues,and its relationship with clinical pathological characteristics was analyzed;the expression of miRNA-29c in each group of cells after transfection was detected by Q-RT-PCR;CCK-8 was used to detect changes in cell proliferation ability;Transwell assays were used to detect changes in the number of transmembrane cells.Results The relative expression level of miRNA-29c in the tissue of laryngeal carcinoma was significantly lower than that in adjacent cancer tissues(P<0.01).The expression level was closely related to different clinical stages,TNM stages and lymphatic metastasis(P<0.05);the expression level of miRNA-29c in the cells of transfected miRNA-29c mimic group was significantly higher than that in the unrelated sequence group and the blank control group(P<0.01),and it can inhibit the proliferation and invasiveness of laryngeal carcinoma Hep-2 cells(P<0.01).Conclusion In laryngeal cancer,miRNA-29c is an inhibitory miRNA.Low expression of miRNA-29c may be an important factor affecting the prognosis of laryngeal cancer;overexpression of miRNA-29c can significantly reduce the proliferation and invasiveness of laryngeal carcinoma Hep-2 cells.Down-regulation of miRNA expression level may be involved in the occurrence,development,invasion and metastasis of laryngeal carcinoma.