基于石墨烯和功能核酸的Ag^+荧光检测方法

Fluorescent Detection of Ag^+Using Graphene and Functional Nucleic Acid

基于核酸适配体错配结合Ag^+的原理,利用石墨烯对单链DNA和双链DNA结合力的不同,设计开发了C-Ag^+-C和石墨烯结合的新型荧光传感器,并建立了一种高灵敏度、快速、便捷的荧光检测方法,实现了turn on形式的Ag^+检测。在优化条件下,检测Ag^+的线性范围为150-400 nmol/L,检出限为23 nmol/L。该方法在饮用水的实验样品中Ag^+加标检测的回收率达到96.6%,具备用于实际样品分析的能力。

Based on the principle of mismatched binding Ag^+of nucleic acid aptamer,using the different binding forces of graphene to single-stranded DNA and double-stranded DNA,a new fluorescent sensor with C-Ag^+-C and graphene binding was designed and developed.A high sensitivity,fast and convenient fluorescence detection method was developed to achieve the Ag^+detection in turn on form.Under the optimization of detection conditions,the turn on fluorescent aptasensor offered a linear detection range of 150-400 nmol/L with a detection limit of 23 nmol/L for Ag^+.The recovery rate of the method was 96.6%for Ag^+-labeled detection in the experimental samples of drinking water,showing the ability to be used for actual sample analysis.