阿魏菇凝集素对小鼠腹腔巨噬细胞免疫活性的影响

Effect of Pleurotus ferulae Lenzi Lectin on Macrophage Immunomodulatory Activity in Mice

凝集素因具有专一识别性、免疫活性及抗癌作用而受到广泛关注。以阿魏菇为原材料,提取阿魏菇凝集素,通过阿魏菇凝集素对小鼠巨噬细胞的作用来评价其免疫调节作用。结果表明:在测定范围,凝集素PFLL增强了巨噬细胞的增殖活性。一氧化氮(NO)、超氧阴离子(O2-)、肿瘤坏死因子(TNF-α)、白介素-1(IL-1)的生成量均呈一定的剂量依赖性和时间依赖性。凝集素显著诱导以上物质分泌的剂量分别为20,100,10,10,30μg/mL和20μg/mL,且在12 h内,几种细胞因子生成量显著增强。值得注意的是,无论是否添加佛波脂(PMA),试验组的O2-生成量均大于对照组。结论:阿魏菇凝集素可以增强巨噬细胞的免疫活性,具有潜在的相关疾病的治疗作用。

Lectins have received extensive attention due to their unique identity,immune activity and anti-cancer effects.The Pleurotus ferulae Lenzi lectin(PFLL)was isolated and purified from Pleurotus ferulae Lenzi.The objective was to evaluate the immunomodulatory effects of PFLL on the activity of macrophages.The results indicate that PFLL increased the proliferative activity,the production of nitric oxide(NO),superoxide anion(O2-),tumor necrosis factor(TNF-α),interleukin-1(IL-1)in a dose-and time-dependent manner.The production of NO,O2-,TNF-α,IL-1 were significantly induced by PFLL at a dose of 20,100,10,10,30,20μg/mL.A time-dependent enhancement showed that the production of NO,O2-,TNF-α,IL-1 were significantly increased within 12 h.O2-production by macrophages from PFLL-treated mice was higher than that of cells from untreated mice.Moreover,cells from both untreated and treated mice responded to phorbol 12-myristate 13-acetate(PMA)treatment;however,the O2-production was higher in the cells from treated mice than that of cells from untreated mice.Our data suggest that the beneficial therapeutic effects of PFLL may be attributed partly to its ability to modulate macrophage immune functions.