水杨酸·甲基茉莉酸与伤诱导对烟草sm-Nvas同源基因表达调控的初步研究

Effects of Salicylic Acid,Methyl Jasmonate and Wound Induction on the Expression of sm-Nvas Homologous Genes in Tobacco

[目的]研究Nicotiana sylvestris和Nicotiana attenuata这2种烟草中sm-Nvas同源基因分别在经水杨酸(SA)、甲基茉莉酸(MeJA)及伤诱导后的表达情况。[方法]采用Trizol法提取经水杨酸、甲基茉莉酸及伤处理后的2种烟草叶片的总RNA,转录成cDNA,利用实时荧光定量PCR检测sm-Nvas同源基因的表达量并进行数据分析。[结果]通过对烟草叶片中sm-Nvas同源基因的表达量分析,结果表明水杨酸、甲基茉莉酸及伤处理能对sm-Nvas同源基因的表达起调控作用。[结论]用SA、MJA和伤处理Nicotiana sylvestris和Nicotiana attenuata烟草,发现sm-Nvas的表达量均有所增加。在处理8 h后其表达量发生了明显的变化,在12~16 h其表达量的增加达到峰值。这对研究烟草的sm-Nvas及其同源基因的功能奠定重要的基础。

[Objective]To study the expression of sm-Nvas homologous genes in two tobaccos,Nicotiana sylvestris and Nicotiana attenuata,after salicylic acid(SA),methyl jasmonate(MeJA) and wounding induction,respectively.[Method]Total RNA was extracted from the two tobacco leaves treated with SA,MeJA and wounding using Trizol method,and then reverse transcribed into cDNA.The expression of sm-Nvas homologous gene was detected by real-time fluorescence quantitative PCR and the data were analyzed.[Result]Based on analysis of the expression level of sm-Nvas homologous genes in tobacco leaves,it showed that salicylic acid,methyl jasmonic acid and wound treatment could regulate the expression of sm-Nvas homologous genes.[Conclusion]After SA,MJA and wounding treatment of Nicotiana sylvestris and Nicotiana attenuata tobacco,the expression of sm-Nvas homologous gene increased.After 8 hours of treatment,all the expression levels changed significantly,and reached the peak at about 12-16 hours.This will lay an important foundation for studying the function of sm-Nvas and its homologous genes in tobacco.