不同预处理对猴头菇菌渣及水稻秸秆水解性能的影响

Effects of Different Pre-treatments on Hydrolysis of Hericium erinaceus Residue and Rice Straw

以猴头菇菌渣和水稻秸秆为原料,以纤维素酶和表面活性剂为水解剂,研究了添加水解剂对还原糖含量、CMC酶活性、底物回收率等的影响。结果表明,添加表面活性剂的纤维素酶降解秸秆纤维素产糖的效率高于未添加表面活性剂的降解产糖效率,纤维素酶降解试验底物回收率大小顺序为TU2>TU1>CK>HOU2>HOU1;猴头菇菌渣降解率远远高于碱处理水稻秸秆,在酶解试验中产糖量大小顺序为HOU2>TU2>TU1>CK>HOU1。菌渣中酶和加入的酶液共同降解底物产糖效率高于其他组,在酶解试验中酶活性大小顺序为HOU1>TU2>TU1>HOU2>CK。纤维素酶降解纯菌渣CMC酶活性最高。

In this study, we used Hericium erinaceous residue and rice straw as raw materials, used cellulase and surfactant as hydrolyzers, the effects of adding hydrolyzers on reducing sugar, CMC enzyme activity and substrate recovery rate were studied.The results showed that the sugar-producing efficiency of cellulase with adding surfactant was higher than that without surfactant in the degradation of straw cellulose.The order of substrate recovery rate in cellulase degradation experiment was TU2 >TU1>CK>HOU2 > HOU1.The degradation rate of H.erinaceus residue was much higher than that of alkali-treated rice straw.In the enzymolysis experiment, the order of sugar production was HOU2> TU2>TU1>CK>HOU1.The sugar-producing efficiency of common degradation substrate of enzyme in the bacterial residue and the added enzyme solution was higher than that of other groups.In the enzymatic hydrolysis experiment, the enzyme activity order was HOU1> TU2> TU1> HOU2>CK.CMC enzyme activity of pure bacterial residue degraded by cellulase was the highest.