硫化物胁迫对刺参(Apostichopus japonicus)幼参的急性毒性效应及抗氧化防御系统的影响

Effects of Sulfide Stress on Acute Toxicity and Antioxidant Defense System of Apostichopus japonicas

以刺参(Apostichopus japonicus)幼参为研究对象,探究了硫化物对刺参的急性毒性效应,试验结果表明硫化物对刺参幼参的24 h、48 h、72 h、96 h的半致死浓度(LC 50)分别为4.397、3.769、3.001、2.678 mg/L,安全浓度(SC)为0.2678 mg/L.研究了在0(对照组)、0.8(低浓度)、1.6 mg/L(高浓度)硫化物浓度下,暴露24 h、48 h、72 h、96 h时刺参肠道中超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性、总抗氧化能力(T-AOC)和总谷胱甘肽(GSH+GSSG)含量的变化.结果显示:在硫化物暴露下,低浓度硫化物处理组刺参幼参T-AOC始终略高于对照组.而高浓度硫化物处理组在24 h时显著高于对照组,随后随时间不断下降,至96 h,显著低于对照组(P<0.05).SOD和CAT活性均在硫化物暴露72 h内不断升高,至72 h,高、低浓度硫化物处理组均显著高于对照组(P<0.05),72 h后变化不大.硫化物处理下高、低浓度组GSSG+GSH含量始终高于对照组(P<0.05).研究结果为探索硫化物胁迫下刺参抗氧化防御机制提供基础参考.

Acute toxic effects of sulfide on juvenile of sea cucumber Apostichopus japonicas are investigated.24 h-LC 50,48 h-LC 50,72 h-LC 50 and 96 h-LC 50 of sulfide for cucumber juvenile is 4.397,3.769,3.001,and 2.678 mg/L,respctively and the safe concentration(SC)is 0.2678 mg/L.Activities of superoxide dismutase(SOD),catalase(CAT),total antioxidant capacity(T-AOC),and content of total glutathione(GSSG+GSH)are investigated in different concentrations of sulfide for 24 h,48 h,72 h and 96 h.The results show that the T-AOC in low-sulfide treatment group is always slightly higher than that in the control group.However,the T-AOC in high-concentration sulfide treatment group is significantly higher than that in the control group at 24h(P<0.05),and then continuously decreases with time.The activities of SOD and CAT in the high and low concentration sulfide treatment groups increase continuously within 72 h of sulfide exposure,and the activity of the two enzymes at 72 h are significantly higher than those in the control group(P<0.05).The content of GSSG+GSH in the low-concentration and high-concentration sulfide treatment groups is always significantly higher than that in the control group(P<0.05).These findings provide basic reference to further elucidate the antioxidant defense mechanism of sea cucumber under sulfide stress.