夹脊电针对兔退变腰椎间盘组织中caspase3、caspase9表达的影响

Effects of Electroacupuncture Stimulation of“Jiaji(EX-B 2)”on Expression of Caspase-3 and Caspase-9 in Rabbit Degenerated Intervertebral Disc

目的:观察夹脊电针对兔退变腰椎间盘组织中caspase-3、caspase-9表达的影响。方法:将20只新西兰兔随机分为正常组(n=5)、假模型组(n=5)、模型组(n=5)、电针组(n=5)。正常组给予正常喂养,不予特殊处理;假模型组实验兔在L 4、L 5椎体间穿入克氏针,不予椎体间加压,不做治疗;模型组在L 4、L 5椎体间穿入两根平行的克氏针,然后在克氏针两端连接加压装置,不做治疗;电针组在成功造模的基础上,施以电针治疗。在造模后第28d各组实验兔取出腰椎间盘组织,并通过免疫组化检测其中caspase3、caspase9表达情况。结果:干预28d后,与正常组同期相比较,假模型组的caspase-3、caspase-9表达差异无统计学意义,模型组caspase-3、caspase-9表达水平均显著升高(均P<0.05);与模型组同期比较,电针组caspase-3、caspase-9表达均显著降低(均P<0.05)。结论:夹脊电针通过下调腰椎间盘组织中caspase-3、caspase-9表达,减少细胞凋亡,从而延缓腰椎间盘退变。

Objective:To observe the effect of electroacupunture at jiaji(EX-B2)on the expression of caspase-3 and caspase-9 in rabbit degenerated intervertebral disc.Method:A total of 20 New Zealand rabbits were randomly divided into normal,sham operation,model,and EA groups(n=5 each).Rabbits in normal group were given natural breeding without any treatment.The rabbits in model group were given the custom-made external device on the bases of two K-wires inserted into the vertebral bodies L4 and L5 parallel to the adjacent study for 28 days.There were nothing else treatment in this group.The rabbits in sham operation group were attached to two K-wires inserted into the vertebral bodies L4 and L5 parallel to the adjacent study without any pressure loading devices or other treatment.The rabbits in EA group were given EA therapy from the first day of the disc degeneration model established.The expression levels of caspase-3 and caspase-9 in the lumbar intervertebral disc(L4-L5)tissues were assayed by immunohistochemistry at 28th day.Results:No significant changes were found between the control and sham groups at any time point for the expression of caspase-3 and caspase-9.Compared to the normal group,caspase-3 and caspase-9 expression levels at 28th day in the model group were apparently increased(all P<0.05).As compared with the model group,the expression levels of caspase-3 ancaspase-9 were remarkably decreased in the EA group at day 28(P<0.05).Conclusion:The treatment of EA at jiaji(EX-B2)has a protective effect on the tissues of the lumbar intervertebral disc in rabbits.Its mechanism of delaying the degeneration of the lumbar intervertebral disc may be related to the down-regulation of caspase-3 and caspase-9.