基于贵金属纳米颗粒构建无酶过氧化氢电化学传感器的研究

Enzyme-free electrochemical sensor for hydrogen peroxide detection based on the electrodeposition of metal nanoparticles

采用一步电化学沉积方法分别将三种常用贵金属纳米颗粒(Au,Ag,Pt)负载于工作电极上,构建了基于纳米薄膜的过氧化氢(H2O2)无酶电化学传感器。通过扫描电子显微镜(SEM)表征证明三种金属纳米颗粒成功修饰在玻碳电极(GCE)表面。通过比较三种金属纳米颗粒检测H2O2的能力,发现Ag纳米粒子具有更优异的催化活性。进一步研究扫描速率和检测电压对Ag/GCE催化性能的影响。电化学实验结果表明,该修饰电极显示出优异的H2O2催化活性以及在优化条件下可以达到0.01~23mmol·L^-1的线性范围,检出限为3.3μmol·L^-1,并将该传感器用于检测吐温80中残留H2O2,为吐温80的质量检查提供了一种简单快捷的方法。

The enzyme-free electrochemical sensor was fabricated for hydrogen peroxide detection by immobilizing three kinds of metal nanoparticles(Ag,Au,Pt)on working electrodes via electrodeposition method.Scanning electron microscope(SEM)images demonstrated that the electrodeposited nanoparticles were densely distributed on the glassy carbon electrode(GCE)surface.Electrocatalytic activity of different nanoparticles was investigated by cyclic voltammetry,and Ag nanomaterials displayed the best electrocatalytic property than other two materials.The effects of scanning rate and detection potential on electrocatalytic performance were further investigated.The results show that the modified electrode exhibited the excellent electrocatalytic activity for specific detection of H2O2.Under the optimum conditions,the sensor can reach the linear range of0.01~23 mmol·L^-1 and the detection limit is3.3μmol·L^-1.The electrochemical sensor is performed to detect the residual hydrogen peroxide in Tween 80,which provides a simple and quick method for the quality inspection of Tween 80.