富勒醇修饰电极的多巴胺电化学传感器研究

Electrochemical sensor for dopamine based on the fullerol modified electrode

以富勒烯和H2O2为原料在强碱条件下采用回流氧化法合成了水溶性富勒醇(C 60(OH)n)。将Nafion溶液与C 60(OH)n超声混合均匀,并修饰到玻碳电极表面,得到Nafion-C 60(OH)n/GCE。电化学实验结果表明,该修饰电极对多巴胺在磷酸盐缓冲液(PBS)中的电化学反应具有显着的电催化作用。在最佳条件下,氧化峰电流与多巴胺浓度在0.2μmol·L^-1~20μmol·L^-1和20μmol·L^-1~100μmol·L^-1范围内具有良好的线性关系,检测限为0.011μmol·L^-1(S/N=3)。此外,该传感器具有良好的重现性和稳定性,并用于人体血清中的多巴胺分析,回收率达96.1%~97.3%。

The water-soluble fullerol(C 60(OH)n)was synthesized by a reflux oxidation method under strong alkaline condition using fullerene and H2O2 the raw materials.Nafion-C 60(OH)n/GCE was obtained by mixing Nafion solution with fullerol prepared in strongly alkaline conditions and modifying it on the surface of the glassy carbon electrode.The results of electrochemical experiments show that the modified electrode has a significant electrocatalytic effect on the electrochemical reaction of dopamine in phosphate buffered saline(PBS).Under the optimal conditions,the oxidation peak current has a good linear relationship with dopamine concentration in the range of 0.2μmol·L^-1~20μmol·L^-1 and 20μmol·L^-1~100μmol·L^-1,and the detection limit is 0.011μmol·L^-1(S/N=3).In addition,the sensor has good reproducibility and stability,and is used for dopamine analysis in human serum with a recovery rate of 96.1%~97.3%.