人源P2Y12基因生物信息学分析

Bioinformatics Analysis of Human P2Y12 Gene

目的:采用生物信息学方法对人源P2Y12基因结构特征进行分析。方法:利用DNAstar、ProtParam、SOPMA、SignalP 4.1、MEGA等5个生物信息学平台,研究人源P2Y12基因和蛋白序列,及其理化性质、二级结构、跨膜结构、信号肽、疏水性、磷酸化和羰基化位点及B细胞抗原表位,并且对其蛋白作用功能和亲缘性进行分析。结果:人P2Y12基因编码区长1029 bp,编码342个氨基酸。人P2Y12蛋白分子式为C 1836 H 2868 N 450 O 476 S 18,分子量为39438.78 u,理化性质稳定,二级结构以α螺旋和无规则卷曲为主,含7个跨膜结构,无信号肽,有33个磷酸化位点和2个N-糖基化位点,存在6个B细胞抗原表位。通过String数据库得到蛋白相关功能和通路,发现P2Y12蛋白在G蛋白偶联相关受体、cAMP信号调节、血小板活化等信号通路中发挥着重要作用,是血小板相关生物过程的重要部分。同源性分析和进化树显示黑猩猩和猕猴与人源P2Y12蛋白相似性最高。结论:利用生物信息学方法对人源P2Y12基因及其蛋白进行研究,为血栓、血小板出血性疾病8(BDPLT8)等疾病的研究和相关药物研发应用提供参考。

Objective:To analyze the human P2Y12 gene by using bioinformatics methods.Methods:By retrieving DNAstar,ProtParam,SOPMA,SignalP 4.1,MEGA and other bioinformatics related platforms,the physical and chemical properties,secondary structure,transmembrane structure,signal peptide,hydrophobicity,phosphate And carbonylation sites and B cell antigen epitopes,and the function and affinity of human P2Y12 gene and related protein sequence were analyzed.Results:The coding region of human P2Y12 gene was 1029 bp in length,encoding 342 amino acids.The molecular formula of human P2Y12 protein is C 1836 H 2868 N 450 O 476 S 18,the molecular weight is 39438.78 u,the physical and chemical properties are stable,the secondary structure is mainlyαhelix and random coil,containing seven transmembrane structures,no signal peptide.The protein has 33 phosphorylation sites and 2 N-glycosylation sites There are 6 B cell epitopes.The protein-related functions and pathways were obtained through the String database.It was found that the P2Y12 protein plays an important role in signal pathways such as G protein-coupled receptors,cAMP signal regulation,and platelet activation,and is an important part of platelet-related biological processes.Homology analysis and evolutionary tree show that chimpanzees and rhesus monkeys have the highest similarity to human P2Y12 protein.Conclusion:The use of bioinformatics methods to study the human P2Y12 gene and its protein provides a reference for the research of thrombosis,blee ding disorder,platelet type 8(BDPLT8)and other diseases and the development and application of related drugs.