响应面法优化苔干多糖的提取工艺及其体外抗氧化研究

Optimization of Extraction Technology of Polysaccharides from Lactuca sativa var.Angustata by Response Surface Methodology and Its Antioxidant Activity in Vitro

目的:优化苔干多糖的提取工艺,并研究其体外抗氧化性。方法:采用单因素结合响应面方法优化苔干多糖的提取工艺;分别采用Sevage法、三氯乙酸(TCA)法、木瓜蛋白酶法进行多糖的脱蛋白研究并进行比较;通过研究苔干多糖对DPPH·、羟基自由基及超氧阴离子自由基的清除能力进行多糖的抗氧化性研究。结果:苔干多糖的最佳提取条件为料液比1∶31、提取温度84℃,提取时间80 min,提取次数为2次,超声功率200 W;相比Sevage法和三氯乙酸法脱蛋白,采用酶法对苔干多糖脱蛋白的效果最佳;随着多糖浓度的升高,多糖对DPPH·、羟基自由基及超氧阴离子自由基的清除能力增强,其中苔干多糖对DPPH·、羟基自由基及超氧阴离子自由基的最大清除率分别为59.65%、49.31%、63.93%。结论:在此优化的工艺条件下苔干多糖提取率最佳,同时苔干多糖具有一定的抗氧化性,此研究将为后续开发苔干多糖类的功能性食品以及研究苔干的营养价值机理等提供参考。

Objective:To optimize the extraction process of polysaccharides from Lactuca sativa var.angustata and study its antioxidant activity in vitro.Methods:Single factor and response surface method were used to optimize the extraction process of polysaccharides;Sevage,trichloroacetic acid(TCA)sedimentation and papain were used to deproteinization of polysaccharides;the antioxidant capacity of polysaccharides was determined by studying the scavenging ability of polysaccharides to DPPH·,hydroxyl radicals and superoxide anion radicals.Results:Single factor and response surface method were used to optimize the extraction process of polysaccharides;Sevage,trichloroacetic acid(TCA)sedimentation and papain were used to deproteinization of polysaccharides;the antioxidant capacity of polysaccharides was determined by studying the scavenging ability of polysaccharides to DPPH·,hydroxyl radicals and superoxide anion radicals.Conclusion:Single factor and response surface method were used to optimize the extraction process of polysaccharides;Sevage,trichloroacetic acid(TCA)sedimentation and papain were used to deproteinization of polysaccharides;the antioxidant capacity of polysaccharides was determined by studying the scavenging ability of polysaccharides to DPPH·,hydroxyl radicals and superoxide anion radicals.