热休克蛋白22减轻转化生长因子β1诱导的成纤维细胞激活

Heat shock protein 22 reduces the activation of cardiac fi broblasts induced by transforming growth factorβ1

目的探讨热休克蛋白22(Hsp22)对TGFβ1刺激的心脏成纤维细胞激活的影响,以及其可能的分子机制。方法分离培养小鼠成体心脏成纤维细胞,采用TGFβ1刺激诱导成纤维细胞激活。采用不同浓度的Hsp22(1,2,4,8,10μg/mL)孵育成纤维细胞24 h,观察其对心脏成纤维细胞活化、增殖和分泌的影响;采用CCK8试剂盒检测细胞增殖活性;采用RT-PCR检测促纤维化因子的转录水平;采用免疫荧光染色检测a-SMA蛋白的表达;采用免疫印迹检测可能的信号蛋白的改变。结果CCK8结果显示,TGFβ1刺激后成纤维细胞增殖明显增加(P<0.05);TGFβ1刺激后成纤维细胞α-SMA表达增多(P<0.05),纤维化相关的基因转录明显增加(P<0.05)。1,2,4,8,10μg/mL的Hsp22均能够显著抑制成纤维细胞的增殖(P<0.05)。8μg/mL和10μg/mL的Hsp22能够抑制α-SMA表达(P<0.05),减少纤维化相关的基因的转录水平(P<0.05)。免疫印迹结果显示TGFβ1刺激后WNT和β-catenin激活水平增加,GSK3β的磷酸化增高,β-catenin的核转位增多(P<0.05);10μg/mL的Hsp22处理能够抑制WNT和β-catenin的激活水平,减少GSK3β的磷酸化表达,减少β-catenin的核转位(P<0.05),减少smad2和smad3的磷酸化水平(P<0.05)。结论Hsp22可以通过抑制WNT/β-catenin信号通路阻断TGFβ1诱导的成纤维细胞活化、增殖和分泌。

Objective To explore the effect of Hsp22 on the activation of cardiac fibroblasts stimulated by TGFβ1 and its possible molecular mechanism.Methods Cardiac fibroblasts of adult mice were isolated and cultured,and stimulated with TGFβ1 to induce fibroblast activation.Fibroblasts were incubated with Hsp22 of different concentrations(1,2,4,8,10μg/mL)for 24 h,and their activation,proliferation and secretion were observed.CCK8 kit was used to detect cell proliferation.RT-PCR was used to detect the transcription of fibrogenic factor.Immunofl uorescence was used to detect the expression ofα-SMA protein.Immunoblotting was used to detect the possible signal protein.Results CCK8 results showed that fibroblast increased significantly after TGFβ1 stimulation(P<0.05).The expression ofα-SMA in fibroblasts and the transcription of fibrosis-related genes increased significantly after TGFβ1 stimulation(P<0.05).Different concentrations(1,2,4,8,and 10μg/mL)of Hsp22 all inhibited the proliferation of fibroblasts significantly((P<0.05).Eightμg/mL and 10μg/mL Hsp22 inhibited the expression ofα-SMA(P<0.05).and reduced the transcription of fibrosis-related genes(P<0.05).Immunoblotting results indicated that after induced by TGFβ1,the expression of WNT andβ-catenin,the phosphorylation level of GSK3β,and the nuclear translocation ofβ-catenin increased(P<0.05).Tenμg/mL Hsp22 inhibited the expression of WNT andβ-catenin,and reduced the phosphorylation of GSK3βthe nuclear translocation ofβ-catenin and the phosphorylation of smad2 and smad3(P<0.05).Conclusions Hsp22 could block TGFβ1-induced fibroblast activation,proliferation and secretion via inhibiting the WNT/β-catenin signaling pathway.