miR-155和SOCS6在结核感染鉴别诊断中的价值

The value of miR-155 and SOCS6 in differentiating tuberculosis infection status

目的:探讨微小RNA155(miR-155)和细胞因子信号抑制分子6(SOCS6)在结核感染鉴别诊断中的价值。方法:病例对照研究。回顾性纳入2017年1至6月来第三军医大学第三附属医院就诊的60例患者,分为活动性肺结核组、潜伏性结核组和其他肺部感染性疾病组,每组各20例。采用荧光定量PCR法检测miR-155与SOCS6基因在不同结核感染状态人群外周血单个核细胞中的表达水平。采用Logistic回归分析获得miR-155与SOCS6联合应用指标(Co-index)。研究miR-155、SOCS6以及Co-index在鉴别结核感染中的价值。绘制受试者工作特征(ROC)曲线,计算ROC曲线下面积(AUC)。统计分析和制图通过MedCalc和GraphPad Prism 8.0软件实现。结果:鉴别结核感染与未感染时,miR-155(AUC=0.663,P=0.031),SOCS6(AUC=0.708,P=0.002)和Co-index(AUC=0.718,P=0.001)鉴别力均不佳。在鉴别活动性感染和潜伏性感染时,miR-155(AUC=0.867,P<0.001)和Co-index(AUC=0.875,P<0.001)鉴别效能高,但两组间差异无统计学意义(Z=0.142,P=0.887);SOCS6(AUC=0.570,P=0.459)鉴别效能低。在鉴别活动性结核感染与非结核其他肺部感染时,Co-index(AUC=0.923,P<0.001)优于SOCS6(AUC=0.723,P=0.007)(Z=2.586,P=0.010),与miR-155(AUC=0.835,P<0.001)比较,差异无统计学意义(Z=1.585,P=0.113)。结论:miR-155是鉴别潜伏性结核感染和活动性结核感染的潜在生物标志物。miR-155联合SOCS6有助于鉴别活动性结核感染与非结核其他肺部感染。

Objective To reveal the significance of microRNA 155(miR-155)level or the suppressor of cytokine signaling 6(SOCS6)level in distinguishing the differentiating tuberculosis(TB)infection.Methods A case-control study was conducted.A total of 60 patients were enrolled in the study retrospectively,including 20 patients with active pulmonary tuberculosis,20 patients with latent tuberculosis and 20 patients with other pulmonary infectious diseases(non-TB infection),who visited The Third Affiliated Hospital of Third Military Medical University from January to June of 2017.The expression level of miR-155 and SOCS6 in peripheral blood mononuclear cells(PBMC)of from these patients were examined by using the quantitative real-time polymerase chain reaction(qPCR)methods.The associated statistics and graphs was utilized to obtain the relationship,which were reflected by the Co-index receiver operating characteristic(ROC)curve or calculating the area under ROC curve(AUC),between the miR-155 and SOCS6 in differentiating tuberculosis infection by using the Logistic Regressive analysis methods,MedCalc and GraphPad Prism 8.0 software.Results Neither of the three index miR-155(AUC=0.663,P=0.031),SOCS6(AUC=0.708,P=0.002)and Co-index(AUC=0.718,P=0.001)was outstanding to distinguish the tuberculosis infection and non-TB infection.Moreover,the miR-155(AUC=0.867,P<0.001)and Co-index(AUC=0.875,P<0.001)were similar sufficient(Z=0.142,P=0.887)to distinguish the active and latent infections.The Co-index(AUC=0.923,P<0.001)was better(Z=2.586,P=0.010)than SOCS6(AUC=0.723,P=0.007),and similar(Z=1.585,P=0.113)to miR-155(AUC=0.835,P<0.001)on the distinguishing active and non-TB infection.Conclusions By performing the qPCR and the correlation-analysis,miR-155 has been considered as a potential biomarker for differentiating latent tuberculosis infection from active tuberculosis infection.Conjoint analysis of miR-155 and SOCS6 benefits the distinguishing active TB infection from other pulmonary infectious diseases.