上海市环境污水监测埃可病毒11型基因特征和循环动态

Analysis of the genetic characterization and circulation dynamic of echovirus 11 based on environmental sewage surveillance in Shanghai,China

目的探讨基于上海市环境污水监测中埃可病毒11型(echovirus 11,Echo11)的基因特征和循环动态变化。方法本研究对上海市2012—2019年环境污水监测获得的Echo11分离株进行了VP1区核酸扩增和序列测定,结合上海市急性弛缓性麻痹(acute flaccid paralysis,AFP)监测系统和健康儿童肠道病毒监测中Echo11分离株以及Echo11参考株进行序列同源性分析和系统发生学分析。结果上海市3个监测系统来源Echo11分离株共有156株,其中2012—2019年环境污水共分离到136株,其流行高峰主要集中在夏秋季。上海市不同来源Echo11分离株进化速度较快,有40.3%存在第54位氨基酸位点突变。上海市不同来源Echo11共有5个基因亚型传播链,即A1、A2、C1、C3和D5基因亚型。2012年,环境污水、AFP病例和健康儿童之间存在A1基因亚型Echo11共循环现象。2012—2015年的优势传播链为A1基因亚型,从2016年开始,优势传播链逐渐变为D5基因亚型。结论环境污水监测真实反映人类肠道病毒(human enterovirus,HEV)的循环状况,对于HEV相关疾病预测预警具有实际意义。

Objective To explore genetic characterization and circulation dynamic changes of Echovirus 11(Echo11)isolated from environmental sewage surveillance.Methods The VP1 coding region of Echo11 isolated from environmental surveillance in Shanghai from 2012-2019 was amplified and sequenced.Homology analysis and phylogenetic analysis on the Echo11 VP1 sequences were performed with reference strains in acute flaccid paralysis(AFP)surveillance,healthy children surveillance and reference strains.Results A total of 156 strains of Echo11 were isolated from different sources in Shanghai,of which 136 strains were isolated from environmental sewage in 2012-2019,with the seasonal peak mainly in summer and autumn;40.3%of the strains from different sources in Shanghai had the 54th amino acid mutation.There are 5 subgenotype of Echo11 from different sources in Shanghai,i.e.A1,A2,C1,C3 and D5.During 2012,there was co-circulation of A1 subgenotype Echo11 among environmental sewage,AFP cases and healthy children.From 2012 to 2015,the dominant transmission chain of Echo11 was A1 subgenotype.Since 2016,the dominant transmission chain of echo11 has gradually changed to D5 subgenotype.Conclusions Environmental surveillance truly reflects the circulation of human enterovirus(HEV),which has practical significance for the prediction and early warning of HEV related diseases.