mariPOC^(■)多重分析法与直接免疫荧光法检测肺炎患儿感染呼吸道病毒结果的比较

Comparison of mariPOC^（■）multiplex assay and direct immunofluorescence assays for detection of respiratory virus in children with pneumonia

目的通过mariPOC^Ⓒ多重分析法与直接免疫荧光法(direct immunofluoresence assay,DFA)检测肺炎患儿中常见的呼吸道病毒,对检测结果进行比较来评估mariPOC^Ⓒ法的检测性能。方法采集肺炎患儿的鼻咽拭子标本,用mariPOC^Ⓒ多重分析法和DFA检测甲型流感病毒、乙型流感病毒、呼吸道合胞病毒、人偏肺病毒、副流感病毒1、2、3型和腺病毒,比较两种方法的检出率和检测结果的一致性。同时用实时荧光PCR法(real-time PCR)为参比方法对两种方法检测不一致的结果进行验证。结果本研究共纳入400名肺炎住院患儿,mariPOC^Ⓒ多重分析法和DFA法总阳性率分别为40.25%、38.25%。mariPOC^Ⓒ多重分析法与DFA法的阳性一致率为92.16%,阴性一致率为91.90%,总一致率为92.00%。其中有32例不一致结果,用实时荧光PCR法进行确认,20个结果与mariPOC^Ⓒ法一致,12个与DFA法一致。mariPOC®多重分析法与real-time PCR相比较,敏感度为93.06%,特异度为100.00%,总符合率达97.00%。结论mariPOC^Ⓒ法与DFA镜检法相比,在肺炎患儿呼吸道病毒检测中的检测性能良好,与real-time PCR相比敏感度、特异度和总符合率良好,mariPOC^Ⓒ多重分析法具有敏感性高、检测快速、智能等明显优势,可有效应用于临床检验。

Objective mariPOC^Ⓒmultianalyte assays and direct immunofluorescence assays(DFA)were used to detect respiratory viruses in children with pneumonia and evaluate the performance of mariPOC^Ⓒmethod by comparing the results.Methods Nasopharyngeal swab samples were tested by both mariPOC^Ⓒand DFA for influenza A and B,respiratory syncytial viruse,adenoviruse,and parainfluenza 1,2,and 3 viruses.Real-time PCR was also used to detect the DNA of FluA、FluB、RSV、PIV 1、2、3 and ADV as reference method,in order to verify the inconsistent result of the above two method.Results Four hundred children with pneumonia were enrolled.The total positive rates of mariPOC^Ⓒand DFA were 40.25%and 38.25%respectively.According to the results,the positive consistency between mariPOC^Ⓒand DFA was 92.16%and the negative consistency was 91.90%,the overall agreement rate was 92.00%.There were 32 samples where mariPOC^Ⓒand DFA gave contradicting results.In real-time PCR confirmation,the result of 20 samples were consistent with mariPOC^Ⓒtest and 12 agreed with DFA.Compared with real-time PCR,the sensitivity of mariPOC^Ⓒwas 93.06%,the specificity was 100.00%,and the total coincidence rate was 97.00%.Conclusions mariPOC^Ⓒmultianalyte assays had good correlation with DFA for detection of respiratory viruses in children with pneumonia.Compared with the reference method real-time PCR,it has better sensitivity,specificity and total coincidence rate.mariPOC^Ⓒmultianalyte assay has the obvious advantages of high sensitivity,fast detection and intelligence,which can be effectively applied to clinical examinations.