红细胞及其去铁胺水平对脑出血大鼠脑水肿的影响

Effects of red blood cell and deferoxamine levels on cerebral edema in rats with cerebral hemorrhage

目的红细胞及其去铁胺水平对脑出血大鼠脑水肿的影响.方法将100只SD大鼠按照随机数字表法分为全血注射组、假手术组及对照组,每组20只,红细胞注射组40只,其中红细胞注射组分为单纯红细胞注射组和红细胞加去铁胺注射组,每组20只.建立动物模型,建模后检测不同时点各组大鼠脑含水量、苏木素-伊红染色结果 及免疫组化染色结果 .结果 建模后6h全血注射组及红细胞注射组出现脑水肿,对照组与假手术组未引起明显的脑水肿,各时间段组间比较差异无统计学意义(P>0.05);全血注射组建模后6 h开始出现水肿,3 d达到高峰,与对照组和假手术组比较差异有统计学意义(P<0.05);红细胞注射组建模6 h便出现水肿,3 d达到高峰,与全血注射组比较差异有统计学意义(P<0.05).建模后各时点红细胞加去铁胺注射组大鼠脑组织含水量显著低于单纯红细胞注射组(P<0.01).全血注射组和红细胞注射组血红素氧合酶阳性细胞数及凋亡细胞数显著多于对照组及假手术组(P<0.01),红细胞注射组显著多于全血注射组(P<0.01).结论红细胞可促使脑出血后水肿形成,注射去铁胺可以显著抑制脑水肿的形成,具有保护作用.溶解红细胞较自体全血产生脑水肿更为严重,和自身所具有毒性介质效果、过氧化机能、凋亡情况相关.

Objective To explore the effects of red blood cell and deferoxamine levels on cerebral edema in rats with cerebral hemorrhage.Methods According to the random number table method,100 SD rats were divided into whole blood injection group,sham operation group and control group,20 rats in each group,and 40 cases in erythrocyte injection group.The erythrocyte injection group was divided into red blood cell injection group and red blood cell plus deferoxamine injection group,20 rats in each group.An animal model was established.After modeling,the brain water content,HE staining results and immunohistochemical staining results of different groups of rats were detected at different time points.Results 6 hours after modeling,cerebral edema occurred in the whole blood injection group and the erythrocyte injection group.The control group and the sham operation group did not cause obvious cerebral edema.There was no significant difference among the groups at each time period(P>0.05).The whole blood injection group began to appear edema 6 hours after modeling and reached a peak at 3 days,which was statistically significant compared with the control group and sham operation group(P<0.05).At each time point after modeling,the water content of the erythrocyte plus deferoxamine injection group was significantly lower than that of the erythrocyte injection group(P<0.01).The number of heme oxygenase positive cells and apoptotic cells in the whole blood injection group and the erythrocyte injection group were significantly higher than those in the control group and sham operation group(P<0.01),and the erythrocyte injection group was significantly more than the whole blood injection group(P<0.01).Conclusions Red blood cells can promote the formation of edema after cerebral hemorrhage.Deferoxamine injection can significantly inhibit the formation of cerebral edema and have a protective effect.The lysis of red blood cells is more serious than autologous whole blood to produce cerebral edema,which is related to its own toxic mediator effect,peroxidation function,and apoptosis.