薄荷-荆芥不同配伍组分对流感病毒的影响

Effect of Different Compatibility of Menthae Haplocalycis Herba-Schizonepetae Herba on Influenza Virus

目的:通过观察薄荷-荆芥不同配伍组分抗流感病毒的药效学差异,探讨薄荷-荆芥最佳配伍组分。方法:SPF级ICR小鼠228只,雌雄各半。随机分为19组,每组12只。各组分别按照薄荷、荆芥黄酮与挥发油组分配比。用乙醚麻醉ICR小鼠,用甲型流感病毒A/PR/8/34(H1N1)10倍半数致死量(LD50)50μL滴鼻感染,制备病毒感染动物模型。正常组以等量生理盐水滴鼻对照。小鼠病毒感染后当天开始灌胃给药,正常组、模型组灌服等量蒸馏水,阳性药组灌胃给予达菲和复方金银花颗粒。每天1次,连续给药7 d。研究薄荷-荆芥不同配伍组分对流感感染小鼠肺指数的影响,苏木素-伊红(HE)染色观察小鼠肺病理组织学变化,免疫组化观察薄荷-荆芥不同配伍组分对甲型流感病毒感染小鼠白细胞介素-6(IL-6),白细胞介素-1β(IL-1β),肿瘤坏死因子-α(TNF-α)表达的影响。结果:采用甲型流感病毒A/PR8/34(H1N1)感染小鼠制备肺感染损伤,与正常组比较,模型组实验末肺质量增大,肺指数显著升高(P<0.01);与模型组比较,达菲组、复方金银花颗粒组、挥发油1-挥发油2组、挥发油1组、挥发油2组给药后实验末肺质量明显降低(P<0.05);与模型组比较达菲组、挥发油1组肺指数显著降低(P<0.01);与模型组比较,复方金银花颗粒组、挥发油1-挥发油2组、挥发油2-黄酮1-黄酮2组、挥发油1-挥发油2-黄酮1组、挥发油1-挥发油2-黄酮2组、挥发油1-挥发油2-黄酮1-黄酮2组、挥发油2组肺指数明显降低(P<0.05);与模型组比较,挥发油1-黄酮1组、挥发油1-黄酮2组、挥发油1-挥发油2组、挥发油1-挥发油2-黄酮1组、挥发油1-挥发油2-黄酮2组、挥发油2组、黄酮2组对IL-6,IL-1β,TNF-α表达显著下降(P<0.01)。结论:不同比例配伍的组分以薄荷挥发油-荆芥挥发油-荆芥黄酮组、薄荷挥发油-荆芥挥发油组、荆芥挥发油组、薄荷挥发油组抗流感病毒效果较好。

Objective:To research optimum compatibility components ratio for the anti-influenza virus effect of different compatibility of Menthae Haplocalycis Herba-Schizonepetae Herba.Method:The 228 SPF grade ICR rats of half male and half female were randomly divided into 19 groups,with 12 rats in each group.In each group,the ratio of flavonoids and volatile oil was determined.ICR rats were anesthetized with ether and infected with influenza A virus A/PR/8/34(H1N1)with A10-fold lethal dose(LD50)50μL nasal drip infection.Than the blank control group was treated with the same amount of normal saline nasal drip control,and the rats were given the drug by gavage on the day after virus infection,the blank group and model group were given the same amount of distilled water,and the positive control group were given Oseltamivirphosphate and compound Jinyinhua granules by gavage,and once a day for 7 days.Different compatibility of volatile oil and flavonoids in Menthae Haplocalycis Herba and Schizonepetae Herba are applied to rats infected with influenza A/PR8/34(H1N1)virus to study the pharmacodynamic screening by lung index of influenza-infected mice and histopathological examination.And antiviral effects as well as the expression of interleukin-6(IL-6),interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α)is studied.Result:Lung infection injury was prepared by infecting mice with influenza A virus A/pr8/34(H1N1).Lung mass increased at the end of the experiment in the model group,and lung index was compared with that in the blank group(P<0.01).And the experimental lung weight of oseltamivirphosphate group,compound Jinyinhua granules group,volatile oil 1-volatile oil 2 group,volatile oil1 group and volatile oil 2 group were compared with that of the model group(P<0.05).Lung index of Oseltamivirphosphate group and volatile oil group was compared with that of model group(P<0.01).Lung index of compound Jinyinhua granules group,volatile oil 1-volatile oil 2 group,volatile oil 2-flavonoids 2 group,volatile oil 1-volatile oil 2-flavonoids 1 group,volatile oil 1-volatile oil 2-flavonoids 2 group,volatile oil 1-volatile oil 2-flavonoids 2 group,volatile oil 1-volatile oil 2-flavonoids 2 group and volatile oil 2 group were compared with the model group(P<0.05).And antiviral effect as well as the expression of IL-6,IL-1β,TNF-αis studied.The expression levels of IL-6,IL-1β,TNF-αin volatile oil 1-flavonoids 1 group,volatile oil 1-flavonoids 2 group,volatile oil 1-volatile oil 2 group,volatile oil 1-volatile oil 2-flavonoids 1 group,volatile oil1-volatile oil 2-flavonoids 2 group,volatile oil 2 groups,flavonoids 2 groups were decreased,compared with the model group(P<0.01).Conclusion:It is found that different combinations have better biological effects and good antiviral effects.This study explores the synergies compatibility rule and mechanism of action on Menthae Haplocalycis Herba and Schizonepetae Herba pairs,which will not only develop the theory of Chinese medicine compatibility but also contribute to laying a foundation for the innovation of the influenza virus drug development.