药用鸡矢藤药材化学成分的UPLC-Q-TOF-MS分析

UPLC-Q-TOF-MS Analysis of Chemical Constituents of Paederiae Herba for Folk Use

目的:对民间使用的4种鸡矢藤类药材(即鸡矢藤Paederia scandens,毛鸡矢藤P.scandens var.tomentosa,狭叶鸡矢藤P.stenophylla及臭鸡矢藤P.foetida)的醇提物化学成分结构进行鉴定,比较4种鸡矢藤醇提物中化学成分种类与含量间的差异。方法:采用超高效液相色谱-串联四极杆-飞行时间质谱(UPLC-Q-TOF-MS)技术对鸡矢藤药材的化学成分进行快速、全面的定性分析,对所得的数据进行人工解析并进行数据库匹配,进而确定4种鸡矢藤化学成分之间的差异。结果:从4种鸡矢藤中共鉴定出23个化合物,包括鸡矢藤苷、鸡矢藤苷酸、车叶草苷、车叶草苷酸、鸡矢藤苷酸甲酯、水晶兰苷等12个环烯醚萜苷类成分,1-O-咖啡酰奎宁酸、绿原酸、隐绿原酸等5个奎宁酸类衍生物,槲皮素-3-O-芸香糖-7-O-葡萄糖苷、槲皮素-3-O-葡萄糖-7-O-木糖苷、芦丁等5个黄酮苷类化合物及1个其他简单化合物金线莲苷。结论:4种鸡矢藤类药材化学成分的UPLC-Q-TOFMS分析中响应强度最高的4个峰为鸡矢藤苷、鸡矢藤苷酸、车叶草苷及车叶草苷酸,均为环烯醚萜苷类成分。

Objective:The chemical constituents of ethanol extracts from Paederia scandens,P.scandens var.tomentosa,P.stenophylla and P.foetida used in folk were identified.And the differences of chemical constituents among the four kinds of ethanol extracts were compared.Method:The chemical constituents of four species of Paederia were qualitatively and rapidly analyzed by UPLC-Q-TOF-MS.The data were analyzed manually and matched through databases to determine the differences among the chemical constituents of four species of Paederia.Result:Twenty-three compounds were identified from four species,including 12 iridoid glycosides,such as paederoside,paederosidic acid,asperuloside,asperulosidic acid,paederosidic acid methyl ester and monotropein.5 quinic acid derivatives,such as 1-O-caffeoylquinic acid,chlorogenic acid,cryptochlorogenic acid,and 5 flavonoid glycosides,such as quercetin-3-O-rutinoside-7-Oglucoside,quercetin-3-O-rutinoside-7-O-xyloside,rutin and simple compoundkinsenoside.Conclusion:Based on the UPLC-Q-TOF-MS analysis of the chemical components of four kinds of Paederia,the four peaks with the highest response intensity were paederoside,paederosidic acid,asperuloside and asperulosidic acid,all of which were iridoid glycosides.