嗜盐真菌Aspergillus montevidensis生物特性研究

Biological Characteristic Research of Halophilic Fungus Aspergillus montevidensis

【目的】从形态学角度对分离自陕北花马盐湖的嗜盐真菌Aspergillus montevidensis生物特性进行了初步探究,为下一步耐盐基因的筛选及新型耐盐农作物的获得提供理论依据。【方法】将嗜盐真菌A.montevidensis分别接种于不同碳源(淀粉、纤维素、麦芽糖、蔗糖、葡萄糖)、不同氮源(硝酸铵、氯化铵、酵母粉、蛋白胨、硝酸钾)以及不同碳氮比培养基中培养,研究高盐环境中不同碳源、不同氮源以及不同碳氮比对嗜盐真菌A.montevidensis生长速率、菌丝体干质量、菌落形态以及显微形态特征的影响。【结果】嗜盐真菌A.montevidensis在盐浓度为1.5 mol/L,以蔗糖为碳源、蛋白胨为氮源的培养基上生长较快且生长状况最好,在此条件下生长速率可达0.63 cm/d,菌丝体干质量达3.05 g/L。最佳碳氮比为10∶1。【结论】嗜盐真菌A.montevidensis的耐盐受不同碳氮源的影响。

【Objective】Biological characteristic of halophilic fungus Aspergillus montevidensis isolated from Huama salt lake in northern Shaanxi was analyzed,which provided theoretical basis for the next step in the screening of salt-tolerant genes and the acquisition of new salt-tolerant crops.【Method】Halophilic fungus A.montevidensis was inoculated into media respectively consisting of different carbon sources(starch,cellulose,maltose,sucrose,glucose),different nitrogen sources(ammonium nitrate,ammonium chloride,yeast powder,peptone,potassium nitrate),and as well as different C/N ratio.Influences of these gradient conditions on the growth rate,dry weight,colony morphology,and microscopic characteristics of the halophilic fungus A.montevidensis were reported.【Result】The growth rate of halophilic fungus A.montevidensis was up to 0.63 cm/d and dry weight was up to 3.05 g/L in the medium with salt concentration of 1.5 mol/L,sucrose as carbon source and peptone as nitrogen source.The optimal C/N ratio was 10∶1.【Conclusion】The salt tolerance of halophilic fungus A.montevidensis was affected by different carbon and nitrogen sources.