表面改性的碳纤维增强聚醚醚酮种植体的制备及其性能研究

The study of biological properties of surface modified carbon fiber reinforced polyetheretherketone implant materials

目的研究氨基表面改性对不同比例的碳纤维增强聚醚醚酮种植体的理化性能和成骨性能的影响。方法通过控制碳纤维的含量制备不同比例的碳纤维增强聚醚醚酮,采用低温等离子体技术在材料表面引入氨基功能团进行改性。通过万能试验机检测材料的力学性能,扫描电镜、水接触角、X射线光电子能谱检测材料的理化性能。通过成骨细胞的黏附、增殖、碱性磷酸酶活性检测,分析氨基改性对材料的成骨性能影响。结果碳纤维的加入增强了聚醚醚酮的力学性能,且30%碳纤维增强聚醚醚酮的力学性能最佳;样品经改性后表面成功引入了氨基团,水接触角明显降低(P<0.05);成骨细胞在改性样品表面培养6h后伸展良好,有板状伪足向四周伸展,且黏附数量多于未改性样品组。在培养1、7天后,氨基改性明显促进了成骨细胞的增殖(P<0.05)。培养7天和14天后,氨基改性明显提高了成骨细胞的ALP活性(P<0.05)。结论碳纤维与聚醚醚酮结合良好,形成碳纤维增强聚醚醚酮复合材料;通过低温等离子体技术,可成功在其表面引入氨基团,显著提高了材料的亲水性能和成骨性能。

%Objective To study the effects of amino group surface modification on the physicochemical properties and osteogenic properties of different proportions of carbon fiber reinforced polyetheretherketone(CF-PEEK)implants.Methods CF-PEEK with different proportions were prepared by controlling the content of carbon fibers,and modified by low temperature plasma technology to introduce amino groups on the surface.The physical and chemical properties of the material were tested by scanning electron microscopy,water contact angle and X-ray photoelectron spectroscopy.The mechanical properties of the material were tested by universal testing machine.The effects of amino modification on the biological properties of osteoblasts were analyzed by detecting the adhesion,proliferation and alkaline phosphatase activity of osteoblasts.Results The mechanical properties of PEEK were enhanced by adding carbon fibers,and the 30%CF-PEEK possessed the better mechanical properties.Amino groups were successfully introduced into the surface of the modified samples,and the water contact angle was significantly reduced(P<0.05);the mechanical properties of PEEK were significantly enhanced by the addition of carbon fibers.After 6 hours of culture,the osteoblasts extended well on the surface of the modified samples,with platelike pseudopods extending all around,and the adherence was more than that of the unmodified samples.After culture for 1 and 7 days,amino modification significantly promoted the proliferation of osteoblasts(P<0.05).After 7 days and 14 days of culture,the activity of ALP in osteoblasts was significantly increased by amino modification(P<0.05).Conclusion Carbon fibers and PEEK are well bonded to form carbon fibers reinforced PEEK composites.By low temperature plasma technology,amino groups could be successfully introduced on the surface of PEEK composites,which significantly improves the hydrophilicity and biological properties of the materials.