KIF20A对胶质瘤细胞增殖、迁移和侵袭、凋亡及细胞周期分布的影响

Effects of KIF20A on the proliferation,migration and invasion,apoptosis and cell cycle distribution of glioma cells

目的:研究KIF20A对胶质瘤U87细胞系增殖、侵袭、迁移、凋亡及细胞周期分布的影响。方法:利用RNA干扰技术下调胶质瘤细胞系U87中KIF20A的表达。RT-qPCR和Western blot分别检测KIF20A在mRNA和蛋白水平上的表达。CCK-8实验检测U87细胞增殖能力的变化,Transwell实验检测U87细胞迁移及侵袭能力的变化,流式细胞术检测U87细胞凋亡及细胞周期的变化。结果:RT-qPCR和Western blot结果显示siRNA-KIF20A显著下调KIF20A的表达,CCK-8和Transwell实验显示下调KIF20A的表达显著抑制了U87细胞的增殖、迁移及侵袭能力,流式细胞术结果显示下调KIF20A的表达显著促进了U87细胞的凋亡,诱导细胞周期阻滞在G0/G1期。结论:下调KIF20A的表达抑制胶质母细胞瘤细胞的增殖、迁移及侵袭,促进胶质母细胞瘤细胞的凋亡并诱导细胞周期G0/G1期阻滞。

Objective:To study the effect of KIF20 A on proliferation,invasion,migration,apoptosis and cell cycle distribution of human glioma cell line U87.Methods:RNA interference technique was used to down-regulate the expression of KIF20 A in glioma cell line U87.RT-qPCR and Western blot were used to detect the expression of KIF20 A at mRNA and protein levels,respectively.The change of proliferation ability of U87 cells was detected by CCK-8 experiment.The change of migration and invasion ability of U87 cells was detected by Transwell experiment,and the change of apoptosis and cell cycle of U87 cells was detected by flow cytometry.Results:The results of RT-qPCR and Western blot showed that siRNA-KIF20 A significantly reduced the expression of KIF20 A.The CCK-8 and Transwell experiments showed that down-regulation of KIF20 A expression significantly inhibited the proliferation,migration and invasion of U87 cells.Flow cytometry showed that down-regulation of KIF20 A expression significantly promoted apoptosis of U87 cells and induced cell cycle arrest in G0/G1 phase.Conclusion:Down-regulation of KIF20 A expression inhibits proliferation,migration and invasion of glioblastoma cells,promotes apoptosis of glioblastoma cells and induces cell cycle G0/G1 phase arrest.