中间轴孔珊瑚白化病病原菌的分离与鉴定

Isolation and identification of pathogen Vibrio alginolyticus from Acropora intermedia suffering from bleaching

人工繁育的中间轴孔珊瑚(Acropora intermedia)出现白化病症状,且白化范围呈蔓延趋势,严重的甚至出现死亡。为探明此次养殖中间轴孔珊瑚白化病的病因,对患病珊瑚进行了病原学研究。从患病珊瑚基部病灶部位分离得到一株优势菌株JU-V039,该菌株经Biolog生理生化分析和16S rRNA基因发育进化树分析,鉴定为溶藻弧菌(Vibrio alginolyticus)。再经人工回接感染,证实溶藻弧菌是引起此次珊瑚白化病的致病原。药物敏感实验显示,该菌株对13种检测抗菌药物的耐药率为15.4%,其中对利福平、复方新诺明、链霉素、红霉素、左氧氟沙星、头孢噻肟、卡那霉素、四环素、萘啶酸和氯霉素10种实验药物表现敏感(S);对青霉素G和氨苄西林表现耐药(R);对多粘菌素B则表现中介(I)。溶藻弧菌与此次养殖中间轴孔珊瑚白化病的发生有直接关系,研究结果有助于进一步了解珊瑚白化病的致病机理,并给出科学有效的疾病控制方案。

Acropora intermedia in artificial breeding has albinism symptoms,which are increasingly serious,even leading to death.In order to find out the cause,we isolated a dominant strain JU-V039 from the basal lesion of the diseased coral,which was identified as Vibrio alginolyticus by Biolog system and 16 S rRNA gene phylogenetic analysis.After artificial infection,V.alginolyticus was confirmed to be the pathogen of the coral bleaching.According to the drug susceptibility test,the resistance rate of the isolate strain JU-V039 to the 13 selected antimicrobial agents was 15.4%,and it was sensitive to 10 drugs of rifampicin,sulfamethoxazole,streptomycin,erythromycin,levofloxacin,cefotaxime,kanamycin,tetracycline,nalidixic acid and chloramphenicol,but resistant to penicillin G and ampicillin,and intermediate to polymyxin B.Thus,V.alginolyticus is directly related to the occurrence of coral bleaching in the breeding of A.intermedia,and the results help to further understand the pathogenic mechanism of coral bleaching and put forward an effective control method.