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MEX3A在非小细胞肺癌中的表达与功能研究 被引量:2

Expression level and function of MEX3A in non-small cell lung cancer
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摘要 目的:探究MEX3A基因在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达水平及沉默MEX3A基因后对NSCLC增殖、侵袭、迁移和凋亡及周期的影响.方法:通过mRNA转录组分析及癌症基因图谱(The Cancer Genome Atlas,TCGA)数据库信息分析,筛选出在NSCLC中显著高表达的MEX3A基因.通过qRT-PCR检测MEX3A在4种常见NSCLC细胞系中的mRNA表达水平,发现MEX3A在A549和NCI-H292中显著高表达.利用RNA干扰技术(RNA interference,RNAi)沉默A549和NCI-H292中MEX3A的表达,CCK8及Transwell试验检测沉默MEX3A表达后对NSCLC增殖、侵袭及迁移的影响,流式细胞术分析沉默MEX3A表达后对A549和NCI-H292细胞周期及凋亡的影响.结果:MEX3A在A549和NCI-H292中高表达.沉默MEX3A后NSCLC增殖、侵袭及迁移被显著抑制,细胞周期阻滞于G2/M期,促进细胞凋亡.结论:MEX3A作为NSCLC的促癌基因,参与了NSCLC的生长及增殖过程. Objective:To investigate the expression of MEX3A in non-small cell lung cancer(NSCLC)cells and the effects of MEX3A knockout on cell cycle,proliferation,invasion,migration,and apoptosis.Methods:We screened out MEX3A,which was significantly highly expressed,by mRNA microarray and The Cancer Genome Atlas(TCGA)database information analysis.Quantitative real-time polymerase chain reaction(qRT-PCR)was used to detect the expression level of MEX3A in NSCLC cells.A549 and NCI-H292 cells showed high expression of MEX3A.RNA interference(RNAi)method was used to silence MEX3A in A549 and NCI-H292 cells.Cell counting kit-8(CCK8)and Transwell assays were used to determine the effects of MEX3A knockout on proliferation,invasion,and migration in NSCLC cells.Flow cytometry was used to analyze the effects of MEX3A knockout on cell cycle and apoptosis.Results:A549 and NCI-H292 cells showed high expression of MEX3A.After silencing MEX3A,the proliferation,invasion,and migration of NSCLC cells were significantly decreased,while the cell cycle was blocked at G2/M phase and its apoptotic ability was weakened.Conclusions:MEX3A may play an important role as an oncogene in the growth and proliferation of NSCLC cells.
作者 朱贝 郭小朋 董天祺 肖春杰 Bei Zhu;Xiaopeng Guo;Tianqi Dong;Chunjie Xiao(Medicine Clinical Laboratory,Huangshi Clinical College of Hubei University of ChineseMedicine,Huangshi Hospital of TCM(Infectious Disease Hospital),Huangshi 435000,China;Yunnan University School of Medicine,Kunming 650091,China)
出处 《中国肿瘤临床》 CAS CSCD 北大核心 2020年第15期763-768,共6页 Chinese Journal of Clinical Oncology
基金 国家自然科学基金项目(编号:81860499)资助。
关键词 MEX3A 非小细胞肺癌 基因沉默 促癌基因 muscle excess-3 A(MEX3A) non-small cell lung cancer(NSCLC) gene silencing oncogene
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