滩羊毛色候选基因MC1R和TCF25的mRNA表达量定量分析

Quantitative analysis of mRNA expression of Tan sheep coat color candidate genes MC1R and TCF25

为了探究影响哺乳动物毛色的黑色素皮质受体-1基因(MC1R)和转录因子25基因(TCF25)与滩羊毛色的关系,本研究利用实时荧光定量PCR(qRT-PCR)技术,对15只不同毛色滩羊(纯白、褐斑、黑斑)皮肤组织中MC1R基因和TCF25基因的mRNA表达量进行研究。结果显示,MC1R基因和TCF25基因在3种不同毛色滩羊中均有表达。MC1R基因在不同毛色滩羊皮肤组织中表达量为黑斑>褐斑>纯白,其中黑斑组极显著高于纯白组(P<0.01),显著高于褐斑组(P<0.05),褐斑组显著高于纯白组(P<0.05);TCF25基因在不同毛色滩羊皮肤组织中表达量为纯白>褐斑>黑斑,组间差异不显著(P>0.05)。试验表明了目的基因表达量与滩羊毛色间的关系,为今后滩羊毛色选育与品种改良提供参考。

In order to explore the relationship between the melanocortin-1-receptor gene(MC1R)and transcription factor 25 gene(TCF25),which affect the coat colors of mammals,and the coat colors of Tan sheep,real-time fluorescent quantitative PCR(qRT-PCR)technique was used to study the mRNA expression of MC1R gene and TCF25 gene in skin tissues of 15 Tan sheep with different coat colors(pure white,brown spot and black spot).The results showed that MC1R gene and TCF25 gene were expressed in three different kinds of coat colors of Tan sheep.Expression quantity of MC1R gene in skin tissues of different coat colors of Tan sheep was black spot>brown spot>pure white.The black spot group was significantly higher than the pure white group(P<0.01),the brown spot group was significantly higher than the brown spot group(P<0.05),and the brown spot group was significantly higher than the pure white group(P<0.05).The expression quantity of TCF25 gene in skin tissues of different coat colors of Tan sheep was pure white>brown spot>black spot,with no significant difference among groups(P>0.05).The results showed the relationship between the expression level of target gene and the coat colors of Tan sheep,and provided references for the color breeding and improvement of Tan sheep in the future.