Tween 80作用下芘降解细菌Pyr9在三叶草中的定殖及效能

Effects of Tween 80 on colonization and performance of pyrene-degrading strain Pyr9 in Trifolium repens L.

为明确非离子型表面活性剂作用下多环芳烃(PAHs)降解细菌在植物中的定殖和效能,以Tween 80、芘降解细菌Mycobacterium sp.Pyr9和三叶草作为供试对象,通过温室土培试验,探讨不同含量的Tween 80对菌株Pyr9在三叶草中定殖、分布和效能的影响。结果表明:三叶草中菌株Pyr9的数量随着Tween 80含量的增加呈先升后降的趋势,Tween 80含量为100 mg·kg-1时其数量最高。高剂量Tween 80(≥500 mg·kg-1)显著抑制了三叶草的生长,但Pyr9的定殖有效减弱了该抑制作用。添加不同剂量的Tween 80皆可促进三叶草对芘的吸收,而Pyr9的定殖可显著降低三叶草中的芘含量。此外,添加Tween 80可在一定程度上增强三叶草和Pyr9联合作用下土壤中芘的去除效率。研究表明,适当剂量Tween 80的施用有效促进了菌株Pyr9在三叶草中的定殖和效能,并增强了土壤和植物中芘的去除效率。

To investigate the colonization and performance of PAH-degrading bacterium in plants under nonionic surfactants, a series of greenhouse container experiments were performed with Tween 80, pyrene-degrading bacterium Pyr9, and white clover(Trifolium repens L.)as test subjects. The results showed that the cell counts of Pyr9 colonized in white clover increased initially and later decreased with the increase in Tween 80 content. Maximum count was observed when the content of Tween 80 was 100 mg·kg-1. A high content of Tween 80(≥500 mg·kg-1)significantly inhibited the growth of white clover, but the inhibition was weakened by Pyr9 colonization. The addition of Tween 80 enhanced the pyrene uptake of white clover from soil, while the inoculation of Pyr9 significantly reduced the pyrene content in white clover. Furthermore, Tween 80 enhanced the removal efficiency of pyrene from soil under the combination of white clover and Pyr9.Proper dosage of Tween 80 promoted the colonization and performance of pyrene-degrading strain Pyr9 in white clover and enhanced the removal efficiency of pyrene in soil and plants. The results not only provide a theoretical basis for the safe production of agricultural products in PAH-contaminated areas, but also provide technical support for the"production while remediation"of PAH-contaminated soil.