利用UHPLC-ESI-MS/MS法测定川陈皮与其混伪品中的黄酮类成分

Analysis of flavonoids in Chuan Citrus Reticulata Pericarpium and its adulterant using UHPLC-ESI-MS/MS

为研究区分川陈皮与其常见混伪品,采用UHPLC-MS/MS对川陈皮与其常见混伪品的黄酮类成分进行分析,并利用基于代谢物信息公共数据库对UHPLC-MS/MS检测的一级谱、二级谱数据进行定性分析,并采用主成分分析(PCA)、正交偏最小二乘判别分析(OPLS-DA)以及聚类分析三种方法对化合物进行分析。依靠数据库共鉴定出228种黄酮类化合物,其中多酚12种、花青素21种、黄酮类132种、黄酮醇36种、黄烷酮20种、异黄酮7种,川陈皮中鉴定出特有化合物15种,混伪品中鉴定出特有化合物16种,通过PCA、OPLS-DA以及聚类分析均可将川陈皮与其混伪品准确地区分开来。结果表明基于UHPLC-ESI-MS/MS技术的代谢组分析方法可有效区分川陈皮与其混伪品,可以作为一种川陈皮与混伪品区分以及差异性评价的有效方法,并为其它中药材品种的真伪鉴别以及质量评控提供参考。

In order to distinguish Chuan Citrus Reticulata Pericarpium(CCRP)from its adulterant,the flavonoids in CCRP and its adulterant were determined by UHPLC-MS/MS.The data of primary and secondary spectra detected by UHPLC-MS/MS were qualitatively analyzed based on the public metabolomics database,and the compounds were analyzed by principal component analysis(PCA),partial least squares-discriminant analysis(OPLS-DA)and cluster analysis.Finally,a total of 228 flavonoids compounds including 132 flavones,36 flavonols,20 flavanones,21 anthocyanins,7 isoflavones,and 12 polyphenols were identified by database searching,of which 15 specific compounds were identified in CCRP and 16 specific compounds were identified in adulterant.The CCRP and its adulterant could be accurately distinguished by PCA,OPLS-DA and clustering analysis.The experimental results show that the method of metabolomics analysis based on UHPLC-ESI-MS/MS technique can effectively distinguish CCRP from its adulterant,which can be used as an effective method to differentiate CCRP and the adulterant,and the results also provide reference for the authenticity identification and quality evaluation of other traditional Chinese medicine(TCM).