β-榄香烯对结肠癌细胞耐长春新碱的影响

Effects of β-elemene on Vincristine of Colon Cancer Cells

目的:观察β-榄香烯对结肠癌细胞(SW-480)耐长春新碱(vincristine,VCR)的影响。方法:体外传代培养SW-480细胞,采用CCK8法检测β-榄香烯和VCR对SW-480细胞增殖活力的影响;流式细胞仪检测β-榄香烯和VCR对SW-480细胞凋亡的影响;Western Blot方法分析β-榄香烯对细胞中WEE1蛋白表达的影响,并采用pc DFNA-WEE1质粒过表达技术对SW-480细胞中WEE1蛋白进行过表达。结果:β-榄香烯可以诱导VCR对结肠癌SW-480细胞增殖活力的抑制作用;β-榄香烯+VCR联合组SW-480细胞凋亡率为(35.12±6.39)%,高于VCR干预组的(22.66±5.37)%(P<0.05);β-榄香烯呈剂量、时间依赖性地抑制SW-480细胞中WEE1激酶的表达(P<0.05);pcDFNA-WEE1质粒转染能明显上调SW-480细胞中WEE1激酶表达,并且降低了β-榄香烯+VCR对结肠癌SW-480细胞增殖活力的抑制作用(P<0.05)。结论:β-榄香烯通过抑制WEE1表达,增强VCR对结肠癌细胞化疗敏感性的能力,并促使结肠癌细胞凋亡。

Objective: To observe the effects of β-elemene on vincristine(VCR) of colon cancer cells(SW-480). Methods: SW-480 cells were subcultured in vitro, CCK8 method was used to detect the effects of β-elemene and VCR on proliferation activity of SW-480 cells;flow cytometry to survey their influence on the apoptosis of SW-480 cells;Western blot method to analyze the effects of β-elemene on the expressions of cellular WEE1 protein, pcDFNA-WEE1 plasmid overexpression technique to perform the overexpression of WEE1 protein in SW-480 cells. Results: β-elemene could induce the inhibitory effects of VCR on the proliferation activity of colon cancer cells SW-480 cells;SW-480 cellular apoptosis rate of the combination group of β-elemene+VCR was(35.12 ±6.39)%, higher than(22.66±5.37)% of VCR intervention group(P<0.05);β-elemene inhibited the expressions of WEE1 kinase in SW-480 cells in the dose and time dependent manner(P<0.05);pcDFNA-WEE1 plasmid transfection could obviously raise the expressions of WEE1 kinase in SW-480 cells, and relieve the inhibitory effects of β-elemene+VCR on proliferation activty of SW-480 cells in colon cancer cells(P<0.05). Conclusion: β-elemene could enhance the tolerance of VCR to chemotherapy of colon cancer cells through inhibiting the expressions of WEE1,and promote the apoptosis of colon cancer cells.