三种方法提取的清香木叶精油的抗氧化和抑菌活性研究

Antioxidant and antibacterial activity of essential oil extracted from leaves of the Pistacia weinmannifolia by three methods

以清香木叶为试材,研究超声辅助-共水法(ultrasonic assisted-common water method,UWD)、微波辅助-共水法(microwave assisted-common water method,MWD)、同时蒸馏萃取法(simultaneous distillation extraction,SDE) 3种方法提取的清香木叶精油的主要成分以及对DPPH·和ABTS^+·的清除能力和对枯草芽孢杆菌、金黄色葡萄球菌和大肠杆菌的抑制作用。结果表明,MWD法提取的清香木叶精油的提取率最高为2.03%;UWD法、MWD法、SDE法提取的精油分别鉴定出12、10、10种化合物,占峰面积的95.42%、97.00%、98.01%;3种精油清除DPPH·的能力为UWD精油> MWD精油> SDE精油,清除ABTS^+·的能力为SDE精油> MWD精油> UWD精油;3种精油对枯草芽孢杆菌的抑制作用均高于金黄色葡萄球菌和大肠杆菌,相比MWD和SDE精油,UWD精油具有更好的抑菌和杀菌效果,其对3种菌的最低抑菌浓度(minimum inhibitory concentration,MIC)为0.005、0.02、0.04 g/mL,最低杀菌浓度(minimum bactericidal concentration,MBC)为0.01、0.02、0.04 g/mL。

Using Pistacia weinmannifolia leaves as test materials,the main components,ability to clear DPPH · and ABTS^+·,antibacterial activity on Bacillus subtilis,Staphylococcus aureus and Escherichia coli of essential oil extracted by ultrasonic assisted-common water method (UWD),microwave assisted-common water method (MWD),simultaneous-distillation extraction (SDE) were studied. The results showed that the highest extraction rate of essential oil was 2. 03% achieved by MWD;12,10 and 10 compounds were identified from the essential oils extracted by UWD,MWD and SDE method,which accounted for 95. 42%,97. 00% and 98. 01% of the peak area respectively. The ability of these three essential oils to remove DPPH· was: UWD oil > MWD oil > SDE oil;the ability to clear ABTS^+· was: SDE oil > MWD oil > UWD oil;the inhibitory effects on Bacillus subtilis were higher than those of Staphylococcus aureus and Escherichia coli. Compared with MWD and SDE extracted essential oils,UWD extracted essential oil had better bacteriostasis and bactericidal effects,and its minimum inhibition concentration (MIC) against these three bacteria was: 0. 005,0. 02,0. 04 g/mL,its minimum bactericidal concentration (MBC) was 0. 01,0. 02,0. 04 g/mL.